Shear field around adherent leukocytes as measured by micro-PTV

John E. Pickard, Klaus Ley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Polystyrene micro-particle (bead) tracking velocimetry (μ-PTV) with automatic object detection was used to measure fluid shear rates around adhered leukocytes in venules of the living murine cremaster muscle. Morphology-based automated bead detection was followed by auction-based bead-pair assignment. Beads above or below the objective's depth-of-field were identified and eliminated by a blurriness algorithm which approximated the microscope's point-spread-function as an axially-varying two-dimensional Gaussian. These data allow construction of shear rate and shear stress maps around adherent leukocytes with 0.5 micrometer resolution.

Original languageEnglish (US)
Title of host publicationConference Record of the 41st Asilomar Conference on Signals, Systems and Computers, ACSSC
Pages1298-1302
Number of pages5
DOIs
StatePublished - 2007
Externally publishedYes
Event41st Asilomar Conference on Signals, Systems and Computers, ACSSC - Pacific Grove, CA, United States
Duration: Nov 4 2007Nov 7 2007

Publication series

NameConference Record - Asilomar Conference on Signals, Systems and Computers
ISSN (Print)1058-6393

Conference

Conference41st Asilomar Conference on Signals, Systems and Computers, ACSSC
Country/TerritoryUnited States
CityPacific Grove, CA
Period11/4/0711/7/07

Keywords

  • Automated image analysis
  • Fluid velocity
  • In vivo

ASJC Scopus subject areas

  • Signal Processing
  • Computer Networks and Communications

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