TY - JOUR
T1 - Sida rhomboidea.Roxb extract alleviates pathophysiological changes in experimental in vivo and in vitro models of high fat diet/fatty acid induced non-alcoholic steatohepatitis
AU - Thounaojam, Menaka C.
AU - Jadeja, Ravirajsinh N.
AU - Dandekar, Deven S.
AU - Devkar, Ranjitsinh V.
AU - Ramachandran, A. V.
N1 - Funding Information:
The authors (R.N.J. and M.C.T.) are grateful to University Grants Commission, New Delhi for providing Financial Assistance in the form of RFSMS scholarship. Thanks are also due to Dr. Prasanna S. Ghalsasi, Department of Chemistry, The M.S. University of Baroda, India for kind help in spectroflourometer analysis and to Mr. Hemendra Vekaria, Department of Biochemistry, The M.S. University of Baroda, India for providing CDF-DA dye.
PY - 2012/3
Y1 - 2012/3
N2 - The present study was aim to evaluate protective role of Sida rhomboidea.Roxb (SR) extract against high fat diet/fatty acid induced pathophysiological alterations in experimental model of non-alcoholic steatohepatitis (NASH). Effect of SR extract on plasma levels of markers of hepatic damage, plasma and hepatic lipids, mitochondrial oxidative stress, status of enzymatic and non-enzymatic antioxidants and histopathological changes in liver tissue were evaluated in high fat diet fed C57BL/6J mice. Also, the effect of SR supplementation on lipid accumulation, lipid peroxidation, cytotoxicity and cell viability were evaluated in oleic acid treated HepG2 cells. Supplementation of NASH mice with SR extract prevented high fat diet induced elevation in plasma marker enzymes of liver damage, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status. Further, addition of SR extract to in vitro HepG2 cells minimized oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that SR extract has the potential of preventing high fat/fatty acid induced NASH mainly due to its hypolipidemic and antioxidant activities.
AB - The present study was aim to evaluate protective role of Sida rhomboidea.Roxb (SR) extract against high fat diet/fatty acid induced pathophysiological alterations in experimental model of non-alcoholic steatohepatitis (NASH). Effect of SR extract on plasma levels of markers of hepatic damage, plasma and hepatic lipids, mitochondrial oxidative stress, status of enzymatic and non-enzymatic antioxidants and histopathological changes in liver tissue were evaluated in high fat diet fed C57BL/6J mice. Also, the effect of SR supplementation on lipid accumulation, lipid peroxidation, cytotoxicity and cell viability were evaluated in oleic acid treated HepG2 cells. Supplementation of NASH mice with SR extract prevented high fat diet induced elevation in plasma marker enzymes of liver damage, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status. Further, addition of SR extract to in vitro HepG2 cells minimized oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that SR extract has the potential of preventing high fat/fatty acid induced NASH mainly due to its hypolipidemic and antioxidant activities.
KW - Histopathology
KW - Non-alcoholic steatohepatitis
KW - Oxidative stress,HepG2 cell
KW - Sida rhomboidea.Roxb
UR - http://www.scopus.com/inward/record.url?scp=84856991718&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856991718&partnerID=8YFLogxK
U2 - 10.1016/j.etp.2010.08.009
DO - 10.1016/j.etp.2010.08.009
M3 - Article
C2 - 20832268
AN - SCOPUS:84856991718
SN - 0940-2993
VL - 64
SP - 217
EP - 224
JO - Experimental and Toxicologic Pathology
JF - Experimental and Toxicologic Pathology
IS - 3
ER -