TY - JOUR
T1 - Signal transducer and activator of transcription-3 activation is mediated by N-methyl-D-aspartate receptor and L-type voltage-gated Ca2+ channel during cerebral ischemia in rat hippocampus
AU - Li, Hongchun
AU - Zhang, Quanguang
AU - Zhang, Guangyi
N1 - Funding Information:
This project was supported by the National Nature Science Foundation of China (No. 30070182).
PY - 2003/7/10
Y1 - 2003/7/10
N2 - It has been proved that signal transducer and activator of transcription-3 (STAT3) is expressed and activated following cerebral ischemia/reperfusion in cortex and striatum. Here we investigated the changes in tyrosine phosphorylation and DNA-binding activity of STAT3 in hippocampus in a four-vessel occlusion model of Sprague-Dawley rats. Phospho-STAT3 (in cytoplasm) was enhanced from 5 min and reached its peak level at 10 min of ischemia. While in nucleus, phospho-STAT3 increased from 10 min and then peaked at 30 min of ischemia. Concomitantly, DNA-binding activity of STAT3 demonstrated a similar rule in nucleus extracts. The increased tyrosine phosphorylation and DNA-binding activity of STAT3 in nucleus were blocked by ketamine, an N-methyl-D-aspartate receptor antagonist, or by nifedipine, a L-type voltage-gated Ca2+ channel (L-VGCC) antagonist. These results illustrated that the ionotropic glutamate receptor and L-VGCC are important in mediating STAT3 activation during severe cerebral ischemia.
AB - It has been proved that signal transducer and activator of transcription-3 (STAT3) is expressed and activated following cerebral ischemia/reperfusion in cortex and striatum. Here we investigated the changes in tyrosine phosphorylation and DNA-binding activity of STAT3 in hippocampus in a four-vessel occlusion model of Sprague-Dawley rats. Phospho-STAT3 (in cytoplasm) was enhanced from 5 min and reached its peak level at 10 min of ischemia. While in nucleus, phospho-STAT3 increased from 10 min and then peaked at 30 min of ischemia. Concomitantly, DNA-binding activity of STAT3 demonstrated a similar rule in nucleus extracts. The increased tyrosine phosphorylation and DNA-binding activity of STAT3 in nucleus were blocked by ketamine, an N-methyl-D-aspartate receptor antagonist, or by nifedipine, a L-type voltage-gated Ca2+ channel (L-VGCC) antagonist. These results illustrated that the ionotropic glutamate receptor and L-VGCC are important in mediating STAT3 activation during severe cerebral ischemia.
KW - Cerebral ischemia
KW - L-Type voltage-gated Ca channel
KW - N-Methyl-D-aspartate receptor
KW - Rat
KW - Signal transducer and activator of transcription-3
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U2 - 10.1016/S0304-3940(03)00504-4
DO - 10.1016/S0304-3940(03)00504-4
M3 - Article
C2 - 12809989
AN - SCOPUS:0038180779
SN - 0304-3940
VL - 345
SP - 61
EP - 64
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -