Silencing Breast Cancer Genes Using Morpholino Embedded DNA-Tile-AuNPs Nanostructures

Cansu U. Tunç; Deniz Y. Öztaş; Deniz Uzunoǧlu; Ömer F. Bayrak; Mustafa Çulha

doi:10.1089/hum.2019.119

Silencing Breast Cancer Genes Using Morpholino Embedded DNA-Tile-AuNPs Nanostructures

Cansu U. Tunç, Deniz Y. Öztaş, Deniz Uzunoǧlu, Ömer F. Bayrak, Mustafa Çulha

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

There is an ongoing effort to increase the efficiency of gene delivery for the regulation of diseases-related genes. In this report, we demonstrate the efficiency of a DNA-based nanostructure to deliver morpholino antisense oligonucleotides for the upregulated genes in breast cancer as an alternative to the currently used delivery systems. A DNA-tile structure is constructed by embedding antisense oligonucleotides targeting the HER2 and ERα genes. Then, the sticky ends of the DNA-tile nanostructures are hybridized to gold nanoparticles (AuNPs) coated with the complementary oligonucleotides to enhance their cellular uptake. It is found that the morpholino antisense oligonucleotide embedded DNA-tile-AuNPs structure is 30% more effective than the liposome-based system to deliver morpholinos and induce gene silencing in breast cancer cells. The results of the study suggest that the prepared novel nanostructure is an effective and biocompatible carrier that can be used in in vitro gene silencing studies and can be further pursued in in vivo studies.

Original language	English (US)
Pages (from-to)	1547-1558
Number of pages	12
Journal	Human Gene Therapy
Volume	30
Issue number	12
DOIs	https://doi.org/10.1089/hum.2019.119
State	Published - Dec 2019
Externally published	Yes

Keywords

breast cancer
DNA nanotechnology
DNA-tile
gene silencing
morpholino

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/hum.2019.119

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title = "Silencing Breast Cancer Genes Using Morpholino Embedded DNA-Tile-AuNPs Nanostructures",

abstract = "There is an ongoing effort to increase the efficiency of gene delivery for the regulation of diseases-related genes. In this report, we demonstrate the efficiency of a DNA-based nanostructure to deliver morpholino antisense oligonucleotides for the upregulated genes in breast cancer as an alternative to the currently used delivery systems. A DNA-tile structure is constructed by embedding antisense oligonucleotides targeting the HER2 and ERα genes. Then, the sticky ends of the DNA-tile nanostructures are hybridized to gold nanoparticles (AuNPs) coated with the complementary oligonucleotides to enhance their cellular uptake. It is found that the morpholino antisense oligonucleotide embedded DNA-tile-AuNPs structure is 30% more effective than the liposome-based system to deliver morpholinos and induce gene silencing in breast cancer cells. The results of the study suggest that the prepared novel nanostructure is an effective and biocompatible carrier that can be used in in vitro gene silencing studies and can be further pursued in in vivo studies.",

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author = "Tun{\c c}, {Cansu U.} and {\"O}zta{\c s}, {Deniz Y.} and Deniz Uzunoǧlu and Bayrak, {{\"O}mer F.} and Mustafa {\c C}ulha",

note = "Funding Information: We thank Mine Altunbek, Inci Kurt, and Ayca Z. Aku lke for their helpful discussions. Funding Information: This study was funded by Scientific and Technological Research Council of Turkey (TUBITAK) (Grant number: 115Z426) and Yeditepe University. Publisher Copyright: {\textcopyright} Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.",

year = "2019",

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doi = "10.1089/hum.2019.119",

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volume = "30",

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AU - Öztaş, Deniz Y.

AU - Uzunoǧlu, Deniz

AU - Bayrak, Ömer F.

AU - Çulha, Mustafa

N1 - Funding Information: We thank Mine Altunbek, Inci Kurt, and Ayca Z. Aku lke for their helpful discussions. Funding Information: This study was funded by Scientific and Technological Research Council of Turkey (TUBITAK) (Grant number: 115Z426) and Yeditepe University. Publisher Copyright: © Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.

PY - 2019/12

Y1 - 2019/12

N2 - There is an ongoing effort to increase the efficiency of gene delivery for the regulation of diseases-related genes. In this report, we demonstrate the efficiency of a DNA-based nanostructure to deliver morpholino antisense oligonucleotides for the upregulated genes in breast cancer as an alternative to the currently used delivery systems. A DNA-tile structure is constructed by embedding antisense oligonucleotides targeting the HER2 and ERα genes. Then, the sticky ends of the DNA-tile nanostructures are hybridized to gold nanoparticles (AuNPs) coated with the complementary oligonucleotides to enhance their cellular uptake. It is found that the morpholino antisense oligonucleotide embedded DNA-tile-AuNPs structure is 30% more effective than the liposome-based system to deliver morpholinos and induce gene silencing in breast cancer cells. The results of the study suggest that the prepared novel nanostructure is an effective and biocompatible carrier that can be used in in vitro gene silencing studies and can be further pursued in in vivo studies.

AB - There is an ongoing effort to increase the efficiency of gene delivery for the regulation of diseases-related genes. In this report, we demonstrate the efficiency of a DNA-based nanostructure to deliver morpholino antisense oligonucleotides for the upregulated genes in breast cancer as an alternative to the currently used delivery systems. A DNA-tile structure is constructed by embedding antisense oligonucleotides targeting the HER2 and ERα genes. Then, the sticky ends of the DNA-tile nanostructures are hybridized to gold nanoparticles (AuNPs) coated with the complementary oligonucleotides to enhance their cellular uptake. It is found that the morpholino antisense oligonucleotide embedded DNA-tile-AuNPs structure is 30% more effective than the liposome-based system to deliver morpholinos and induce gene silencing in breast cancer cells. The results of the study suggest that the prepared novel nanostructure is an effective and biocompatible carrier that can be used in in vitro gene silencing studies and can be further pursued in in vivo studies.

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Silencing Breast Cancer Genes Using Morpholino Embedded DNA-Tile-AuNPs Nanostructures

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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