Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability reduction with an oxalate desensitiser

Cynthia K.Y. Yiu; Noriko Hiraishi; Stefano Chersoni; Lorenzo Breschi; Marco Ferrari; Carlo Prati; Nigel N.M. King; David H. Pashley; Franklin R. Tay

doi:10.1016/j.jdent.2005.05.001

Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability reduction with an oxalate desensitiser

Cynthia K.Y. Yiu, Noriko Hiraishi, Stefano Chersoni, Lorenzo Breschi, Marco Ferrari, Carlo Prati, Nigel N.M. King, David H. Pashley, Franklin R. Tay

Oral Biology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Objectives: This study examined the changes in hydraulic conductance and ultrastructure of dentine bonded with simplified etch-and-rinse adhesives to oxalate desensitiser pre-treated acid-etched dentine. Methods: Human dentine disks were acid-etched, treated with an oxalate desensitiser (BisBlock, Bisco), and bonded with One-Step (OS), Single Bond (SB), OptiBond Solo Plus (OB) or Prime and Bond NT (PB). Similar disks from each group were acid-etched, and bonded without oxalate pre-treatment. Hydraulic conductance of the specimens was measured at 20 cm of water pressure and analysed with nonparametric statistical methods. Epoxy resin replicas of the smear layer-covered dentine and bonded dentine were examined with SEM for the extent of fluid transudation. Specimens bonded under perfusion were examined with TEM after tracer immersion. Results: OB and PB exhibited a highly significant reversal of the reduced hydraulic conductance obtained with BisBlock on unbonded acid-etched dentine. Profuse water transudation across the bonded dentine was observed from the replicas. Adhesive interfaces were covered with spherical globules that interfered with dentine hybridization. Conversely, no significant difference in hydraulic conductance was observed in SB, between Bisblock pre-treated, unbonded and bonded acid-etched dentine. Significantly lower hydraulic conductance was shown on application of OS to Bisblock-treated acid-etched dentine. Water transudation was sparse, interfering surface globular structures were absent, and only angular subsurface crystals were seen in the dentinal tubules. Conclusions: Convective water fluxes through dentine may be reduced by applying Bisblock to acid-etched dentine before bonding with One-Step or Single Bond. However, reducing adhesive permeability with the use of oxalate desensitiser is not applicable to low acidity adhesives such as OptiBond Solo Plus and Prime and Bond NT.

Original language	English (US)
Pages (from-to)	106-116
Number of pages	11
Journal	Journal of Dentistry
Volume	34
Issue number	2
DOIs	https://doi.org/10.1016/j.jdent.2005.05.001
State	Published - Feb 2006

Keywords

Dentine perfusion
Etch-and-rinse
Flodec
Oxalate desensitiser
Two-step

ASJC Scopus subject areas

General Dentistry

Access to Document

10.1016/j.jdent.2005.05.001

Cite this

@article{d92af5229da649a981a145aae542c1ed,

title = "Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability reduction with an oxalate desensitiser",

abstract = "Objectives: This study examined the changes in hydraulic conductance and ultrastructure of dentine bonded with simplified etch-and-rinse adhesives to oxalate desensitiser pre-treated acid-etched dentine. Methods: Human dentine disks were acid-etched, treated with an oxalate desensitiser (BisBlock, Bisco), and bonded with One-Step (OS), Single Bond (SB), OptiBond Solo Plus (OB) or Prime and Bond NT (PB). Similar disks from each group were acid-etched, and bonded without oxalate pre-treatment. Hydraulic conductance of the specimens was measured at 20 cm of water pressure and analysed with nonparametric statistical methods. Epoxy resin replicas of the smear layer-covered dentine and bonded dentine were examined with SEM for the extent of fluid transudation. Specimens bonded under perfusion were examined with TEM after tracer immersion. Results: OB and PB exhibited a highly significant reversal of the reduced hydraulic conductance obtained with BisBlock on unbonded acid-etched dentine. Profuse water transudation across the bonded dentine was observed from the replicas. Adhesive interfaces were covered with spherical globules that interfered with dentine hybridization. Conversely, no significant difference in hydraulic conductance was observed in SB, between Bisblock pre-treated, unbonded and bonded acid-etched dentine. Significantly lower hydraulic conductance was shown on application of OS to Bisblock-treated acid-etched dentine. Water transudation was sparse, interfering surface globular structures were absent, and only angular subsurface crystals were seen in the dentinal tubules. Conclusions: Convective water fluxes through dentine may be reduced by applying Bisblock to acid-etched dentine before bonding with One-Step or Single Bond. However, reducing adhesive permeability with the use of oxalate desensitiser is not applicable to low acidity adhesives such as OptiBond Solo Plus and Prime and Bond NT.",

keywords = "Dentine perfusion, Etch-and-rinse, Flodec, Oxalate desensitiser, Two-step",

author = "Yiu, {Cynthia K.Y.} and Noriko Hiraishi and Stefano Chersoni and Lorenzo Breschi and Marco Ferrari and Carlo Prati and King, {Nigel N.M.} and Pashley, {David H.} and Tay, {Franklin R.}",

note = "Funding Information: This study was based on a thesis to be presented by Cynthia K.Y. Yiu to the Faculty of Dentistry, The University of Hong Kong in partial fulfilment of the requirements of the degree of Doctor of Philosophy. The simplified etch-and-rinse adhesives and oxalate desensitiser employed in this study were generously sponsored by the respective manufacturers. This work was supported by grant 10204604/07840/08004/324/01, Faculty of Dentistry, the University of Hong Kong, and by grants DE04911 and DE15306 from the National Institute of Dental Research (P.I. David Pashley). The authors are grateful to Mrs Michelle Barnes and Mrs Zinnia Ng for secretarial support. ",

year = "2006",

month = feb,

doi = "10.1016/j.jdent.2005.05.001",

language = "English (US)",

volume = "34",

pages = "106--116",

journal = "Journal of Dentistry",

issn = "0300-5712",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability reduction with an oxalate desensitiser

AU - Yiu, Cynthia K.Y.

AU - Hiraishi, Noriko

AU - Chersoni, Stefano

AU - Breschi, Lorenzo

AU - Ferrari, Marco

AU - Prati, Carlo

AU - King, Nigel N.M.

AU - Pashley, David H.

AU - Tay, Franklin R.

N1 - Funding Information: This study was based on a thesis to be presented by Cynthia K.Y. Yiu to the Faculty of Dentistry, The University of Hong Kong in partial fulfilment of the requirements of the degree of Doctor of Philosophy. The simplified etch-and-rinse adhesives and oxalate desensitiser employed in this study were generously sponsored by the respective manufacturers. This work was supported by grant 10204604/07840/08004/324/01, Faculty of Dentistry, the University of Hong Kong, and by grants DE04911 and DE15306 from the National Institute of Dental Research (P.I. David Pashley). The authors are grateful to Mrs Michelle Barnes and Mrs Zinnia Ng for secretarial support.

PY - 2006/2

Y1 - 2006/2

N2 - Objectives: This study examined the changes in hydraulic conductance and ultrastructure of dentine bonded with simplified etch-and-rinse adhesives to oxalate desensitiser pre-treated acid-etched dentine. Methods: Human dentine disks were acid-etched, treated with an oxalate desensitiser (BisBlock, Bisco), and bonded with One-Step (OS), Single Bond (SB), OptiBond Solo Plus (OB) or Prime and Bond NT (PB). Similar disks from each group were acid-etched, and bonded without oxalate pre-treatment. Hydraulic conductance of the specimens was measured at 20 cm of water pressure and analysed with nonparametric statistical methods. Epoxy resin replicas of the smear layer-covered dentine and bonded dentine were examined with SEM for the extent of fluid transudation. Specimens bonded under perfusion were examined with TEM after tracer immersion. Results: OB and PB exhibited a highly significant reversal of the reduced hydraulic conductance obtained with BisBlock on unbonded acid-etched dentine. Profuse water transudation across the bonded dentine was observed from the replicas. Adhesive interfaces were covered with spherical globules that interfered with dentine hybridization. Conversely, no significant difference in hydraulic conductance was observed in SB, between Bisblock pre-treated, unbonded and bonded acid-etched dentine. Significantly lower hydraulic conductance was shown on application of OS to Bisblock-treated acid-etched dentine. Water transudation was sparse, interfering surface globular structures were absent, and only angular subsurface crystals were seen in the dentinal tubules. Conclusions: Convective water fluxes through dentine may be reduced by applying Bisblock to acid-etched dentine before bonding with One-Step or Single Bond. However, reducing adhesive permeability with the use of oxalate desensitiser is not applicable to low acidity adhesives such as OptiBond Solo Plus and Prime and Bond NT.

AB - Objectives: This study examined the changes in hydraulic conductance and ultrastructure of dentine bonded with simplified etch-and-rinse adhesives to oxalate desensitiser pre-treated acid-etched dentine. Methods: Human dentine disks were acid-etched, treated with an oxalate desensitiser (BisBlock, Bisco), and bonded with One-Step (OS), Single Bond (SB), OptiBond Solo Plus (OB) or Prime and Bond NT (PB). Similar disks from each group were acid-etched, and bonded without oxalate pre-treatment. Hydraulic conductance of the specimens was measured at 20 cm of water pressure and analysed with nonparametric statistical methods. Epoxy resin replicas of the smear layer-covered dentine and bonded dentine were examined with SEM for the extent of fluid transudation. Specimens bonded under perfusion were examined with TEM after tracer immersion. Results: OB and PB exhibited a highly significant reversal of the reduced hydraulic conductance obtained with BisBlock on unbonded acid-etched dentine. Profuse water transudation across the bonded dentine was observed from the replicas. Adhesive interfaces were covered with spherical globules that interfered with dentine hybridization. Conversely, no significant difference in hydraulic conductance was observed in SB, between Bisblock pre-treated, unbonded and bonded acid-etched dentine. Significantly lower hydraulic conductance was shown on application of OS to Bisblock-treated acid-etched dentine. Water transudation was sparse, interfering surface globular structures were absent, and only angular subsurface crystals were seen in the dentinal tubules. Conclusions: Convective water fluxes through dentine may be reduced by applying Bisblock to acid-etched dentine before bonding with One-Step or Single Bond. However, reducing adhesive permeability with the use of oxalate desensitiser is not applicable to low acidity adhesives such as OptiBond Solo Plus and Prime and Bond NT.

KW - Dentine perfusion

KW - Etch-and-rinse

KW - Flodec

KW - Oxalate desensitiser

KW - Two-step

UR - http://www.scopus.com/inward/record.url?scp=31344439256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31344439256&partnerID=8YFLogxK

U2 - 10.1016/j.jdent.2005.05.001

DO - 10.1016/j.jdent.2005.05.001

M3 - Article

C2 - 15979228

AN - SCOPUS:31344439256

SN - 0300-5712

VL - 34

SP - 106

EP - 116

JO - Journal of Dentistry

JF - Journal of Dentistry

IS - 2

ER -

Single-bottle adhesives behave as permeable membranes after polymerisation. II. Differential permeability reduction with an oxalate desensitiser

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this