Abstract
Background - Emerging data suggest that P-selectin, by controlling adhesion of white blood cells, may be important in limiting the response to vascular injury. Methods and Results - We tested the hypothesis that transient inhibition of P-selectin with either anti-P-selectin monoclonal antibody (mAb) or anti-P-selectin glycoprotein ligand-1 (PSGL-1) mAb would reduce neointima formation in the setting of carotid denudation injury in atherosclerosis-prone apolipoprotein E-/- mice. Neointima formation at 28 days was reduced significantly, by 50% or 80%, by a single injection on the day of injury of 100 or 200 μg P-selectin mAb RB 40.34 and by 55% by a single injection of 100 μg PSGL-1 4RA10 (P≤0.005). In addition, there was a significant reduction in neointimal macrophage content. Conclusions - These findings demonstrate that transient P-selectin or PSGL-1 blockade at the time of arterial injury significantly limits plaque macrophage content and neointima formation in a dose-dependent manner after carotid denudation injury in apolipoprotein E-/- mice.
Original language | English (US) |
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Pages (from-to) | 2244-2249 |
Number of pages | 6 |
Journal | Circulation |
Volume | 107 |
Issue number | 17 |
DOIs | |
State | Published - May 6 2003 |
Externally published | Yes |
Keywords
- Antibodies
- Arteries
- Atherosclerosis
- Cell adhesion molecules
- Inflammation
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Physiology (medical)