TY - JOUR
T1 - Small RNA-Seq analysis reveals miRNA expression dynamics across tissues in the malaria vector, Anopheles gambiae
AU - Bryant, William Bart
AU - Mills, Mary Katherine
AU - Olson, Bradley J.S.C.
AU - Michel, Kristin
N1 - Funding Information:
We thank the anonymous reviewers for their critical review and helpful suggestions with the manuscript. This study was supported by R21AI119544 and R21AI139603 awarded to W.B.B from NIH-NIAID. We thank the University of Kansas Medical Center Genomics Core which is supported by NIH U54 HD 090216, COBRE 5P20GM104936-10, and NIH S10OD021743. This study was also supported by contribution 19-129-J from the Kansas Agricultural Experiment Station. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the funding agencies. We are continually grateful to all members of the Michel lab for mosquito rearing and specifically thank Jordan Marshall Strickler for mosquito tissue dissections.
Publisher Copyright:
Copyright © 2019 Bryant et al.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Malaria continues to be a major global health problem, where disease transmission is deeply linked to the repeated blood feeding nature of the anautogenous mosquito. Given the tight link between blood feeding and disease transmission, understanding basic biology behind mosquito physiology is a requirement for developing effective vector-borne disease control strategies. In the mosquito, numerous loss of function studies with notable phenotypes demonstrate microRNAs (miRNAs) play significant roles in mosquito physiology. While the field appreciates the importance of a handful of miRNAs, we still need global mosquito tissue miRNA transcriptome studies. To address this need, our goal was to determine the miRNA transcriptome for multiple tissues of the pre-vitellogenic mosquito. To this end, by using small RNA-Seq analysis, we determined miRNA transcriptomes in tissues critical for mosquito reproduction and immunity including (i) fat body-abdominal wall enriched tissues, (ii) midguts, (iii) ovaries, and (iv) remaining tissues comprised of the head and thorax. We found numerous examples of miRNAs exhibiting pan-tissue high- or low- expression, tissue exclusion, and tissue enrichment. We also updated and consolidated the miRNA catalog and provided a detailed genome architecture map for the malaria vector, Anopheles gambiae. This study aims to build a foundation for future research on how miRNAs and potentially other small RNAs regulate mosquito physiology as it relates to vector-borne disease transmission.
AB - Malaria continues to be a major global health problem, where disease transmission is deeply linked to the repeated blood feeding nature of the anautogenous mosquito. Given the tight link between blood feeding and disease transmission, understanding basic biology behind mosquito physiology is a requirement for developing effective vector-borne disease control strategies. In the mosquito, numerous loss of function studies with notable phenotypes demonstrate microRNAs (miRNAs) play significant roles in mosquito physiology. While the field appreciates the importance of a handful of miRNAs, we still need global mosquito tissue miRNA transcriptome studies. To address this need, our goal was to determine the miRNA transcriptome for multiple tissues of the pre-vitellogenic mosquito. To this end, by using small RNA-Seq analysis, we determined miRNA transcriptomes in tissues critical for mosquito reproduction and immunity including (i) fat body-abdominal wall enriched tissues, (ii) midguts, (iii) ovaries, and (iv) remaining tissues comprised of the head and thorax. We found numerous examples of miRNAs exhibiting pan-tissue high- or low- expression, tissue exclusion, and tissue enrichment. We also updated and consolidated the miRNA catalog and provided a detailed genome architecture map for the malaria vector, Anopheles gambiae. This study aims to build a foundation for future research on how miRNAs and potentially other small RNAs regulate mosquito physiology as it relates to vector-borne disease transmission.
KW - Malaria
KW - MiRNA
KW - Mosquito
KW - Mosquito tissue
KW - Reproduction
KW - Small RNAs
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U2 - 10.1534/g3.119.400104
DO - 10.1534/g3.119.400104
M3 - Article
C2 - 30846481
AN - SCOPUS:85065758928
SN - 2160-1836
VL - 9
SP - 1507
EP - 1517
JO - G3: Genes, Genomes, Genetics
JF - G3: Genes, Genomes, Genetics
IS - 5
ER -