Spectral marker for Cα damage in beta peptides

Mandy C. Green, Sarah Stelzleni, Joseph S. Francisco

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The work in this article describes a spectral signature for the detection of a Cα radical damaged peptide, which should enable the use of infrared spectroscopic methods to directly monitor oxidative events. Spectra for radical damaged peptides are computed with ab initio methods. The amide bands A, I, II, and III are analyzed for trends in the damage site. The spectral signature is found in a region (i.e., 1700-1620 cm-1) normally void of vibrational absorption bands from stable undamaged beta peptides. An analysis of the vibrational motions of the spectral signature is described. The uniqueness of the spectral signature is explored by an examination and comparison with Cα monoradicals and polyradicals, as well as with other bioradicals that could act as spectral interferences. The identification of unique infrared spectral features for Cα damage could have important implications in diagnostics for beta conformational peptides damaged by oxidative stress processes.

Original languageEnglish (US)
Pages (from-to)550-565
Number of pages16
JournalJournal of Physical Chemistry A
Volume117
Issue number3
DOIs
StatePublished - Jan 24 2013
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Fingerprint

Dive into the research topics of 'Spectral marker for Cα damage in beta peptides'. Together they form a unique fingerprint.

Cite this