Abstract
Aim: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing. Patients & methods: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration. Results: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4°C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples. Conclusion: Archived samples, including DNA stored at 4°C for 20 years, are suitable for comparative studies of DNA methylation.
Original language | English (US) |
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Pages (from-to) | 797-811 |
Number of pages | 15 |
Journal | Epigenomics |
Volume | 10 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2018 |
Externally published | Yes |
Keywords
- methylation profiles
- stability
- storage conditions
ASJC Scopus subject areas
- Genetics
- Cancer Research