Abstract
Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25°C or 37°C caused the release clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride) (10 mM), chloroquin (0.1 mM) and monodansyleadaverine (10 mM) significantly reduced the extent of dissociation.
Original language | English (US) |
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Pages (from-to) | 188-190 |
Number of pages | 3 |
Journal | FEBS Letters |
Volume | 281 |
Issue number | 1-2 |
DOIs | |
State | Published - Apr 9 1991 |
Externally published | Yes |
Keywords
- Amantadine
- Clathrin
- Clathrin coated vesicle
- Hydrophobic amine
- Tromantadine
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology