TY - JOUR
T1 - Streamlined subclass-specific absolute quantification of serum igg glycopeptides using synthetic isotope-labeled standards
AU - Li, Lei
AU - Wang, Shuaishuai
AU - Liu, Ding
AU - Qu, Jingyao
AU - Zhu, He
AU - Chen, Congcong
AU - Gibbons, Christopher
AU - Greenway, Harmon
AU - Wang, Peng
AU - Bollag, Roni J.
AU - Liu, Kebin
N1 - Funding Information:
This work was supported by the National Heart, Lung, and Blood Institute (U54HL142019 to L.L.). We acknowledge Dr. Xi Chen from University of California, Davis for providing the PD26ST plasmid.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/3/16
Y1 - 2021/3/16
N2 - Absolute glycoproteomics quantification has drawn tremendous attention owing to its prospects in biomarker discovery and clinical implementation but is impeded by a general lack of suitable heavy isotope-labeled glycopeptide standards. In this study, we devised a facile chemoenzymatic strategy to synthesize a total of 36 human IgG glycopeptides attached with well-defined glycoforms, including 15 isotope-labeled ones with a mass increment of 6 Da to their native counterparts. Spiking of these standards into human sera enabled simplified, robust, and precise absolute quantification of IgG glycopeptides in a subclass-specific fashion. Additionally, the implementation of the absolute quantification approach revealed subclass-dependent alteration of serum IgG galactosylation and sialylation in colon cancer samples.
AB - Absolute glycoproteomics quantification has drawn tremendous attention owing to its prospects in biomarker discovery and clinical implementation but is impeded by a general lack of suitable heavy isotope-labeled glycopeptide standards. In this study, we devised a facile chemoenzymatic strategy to synthesize a total of 36 human IgG glycopeptides attached with well-defined glycoforms, including 15 isotope-labeled ones with a mass increment of 6 Da to their native counterparts. Spiking of these standards into human sera enabled simplified, robust, and precise absolute quantification of IgG glycopeptides in a subclass-specific fashion. Additionally, the implementation of the absolute quantification approach revealed subclass-dependent alteration of serum IgG galactosylation and sialylation in colon cancer samples.
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U2 - 10.1021/acs.analchem.0c04462
DO - 10.1021/acs.analchem.0c04462
M3 - Article
AN - SCOPUS:85103475790
SN - 0003-2700
VL - 93
SP - 4449
EP - 4455
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -