Studentizing microarray data

Keith A. Baggerly, Kevin R. Coombes, Kenneth R. Hess, David N. Stivers, Lynne V. Abruzzo, Wei Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Microarrays let us measure relative expression levels of thousands of genes simultaneously. While there are a variety of microarray platforms (e.g., radioactively labeled cDNA on nylon membranes, Affymetrix gene chips) we will focus on spotted cDNA arrays. These involve cDNA samples labeled with fluorescent dyes, hybridized to probes spotted by a robotic arrayer onto a glass substrate. Two samples are hybridized to each array, with the two samples labeled with different dyes. The most commonly used dyes are Cy5 (red) and Cy3 (green). Scanning the array produces a greyscale intensity image for each sample; we will refer to these as the two "channels" of the array. While these two samples may both be of interest, it is often the case that there will be one sample of primary interest and the other sample will be from a "control" or "reference" substance (typically a mixture of cell lines).

Original languageEnglish (US)
Title of host publicationComputational and Statistical Approaches to Genomics
PublisherSpringer US
Pages49-59
Number of pages11
ISBN (Electronic)9780387262888
ISBN (Print)0387262873, 9780387262871
DOIs
StatePublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine

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