Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)

Zhi-Chun Ding; Qi Zheng; Bin Cai; Feng Yun Ni; Wen Hao Yu; Xing Chen Teng; Yuan Gao; Fang Liu; Dong Chen; Yang Wang; Hou Ming Wu; Hong Zhe Sun; Ming Jie Zhang; Xiang Shi Tan; Zhong Xian Huang

doi:10.1016/j.jinorgbio.2008.07.007

Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)

Zhi-Chun Ding, Qi Zheng, Bin Cai, Feng Yun Ni, Wen Hao Yu, Xing Chen Teng, Yuan Gao, Fang Liu, Dong Chen, Yang Wang, Hou Ming Wu, Hong Zhe Sun, Ming Jie Zhang, Xiang Shi Tan, Zhong Xian Huang

Biochemistry and Molecular Biology

Research output: Contribution to journal › Short survey › peer-review

12 Scopus citations

Abstract

Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the ⁶CPCP⁹ motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.

Original language	English (US)
Pages (from-to)	1965-1972
Number of pages	8
Journal	Journal of Inorganic Biochemistry
Volume	102
Issue number	11
DOIs	https://doi.org/10.1016/j.jinorgbio.2008.07.007
State	Published - Nov 2008

Keywords

Cell bioassay
Metallothionein (MT)
Mutation
Neuronal growth inhibitory factor (GIF)
Structure-function relationship

ASJC Scopus subject areas

Biochemistry
Inorganic Chemistry

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10.1016/j.jinorgbio.2008.07.007

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Ding, Z.-C., Zheng, Q., Cai, B., Ni, F. Y., Yu, W. H., Teng, X. C., Gao, Y., Liu, F., Chen, D., Wang, Y., Wu, H. M., Sun, H. Z., Zhang, M. J., Tan, X. S., & Huang, Z. X. (2008). Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF). Journal of Inorganic Biochemistry, 102(11), 1965-1972. https://doi.org/10.1016/j.jinorgbio.2008.07.007

Ding, Z-C, Zheng, Q, Cai, B, Ni, FY, Yu, WH, Teng, XC, Gao, Y, Liu, F, Chen, D, Wang, Y, Wu, HM, Sun, HZ, Zhang, MJ, Tan, XS & Huang, ZX 2008, 'Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)', Journal of Inorganic Biochemistry, vol. 102, no. 11, pp. 1965-1972. https://doi.org/10.1016/j.jinorgbio.2008.07.007

@article{e0ed994320f8477aad2641b65518c712,

title = "Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)",

abstract = "Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the 6CPCP9 motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.",

keywords = "Cell bioassay, Metallothionein (MT), Mutation, Neuronal growth inhibitory factor (GIF), Structure-function relationship",

author = "Zhi-Chun Ding and Qi Zheng and Bin Cai and Ni, {Feng Yun} and Yu, {Wen Hao} and Teng, {Xing Chen} and Yuan Gao and Fang Liu and Dong Chen and Yang Wang and Wu, {Hou Ming} and Sun, {Hong Zhe} and Zhang, {Ming Jie} and Tan, {Xiang Shi} and Huang, {Zhong Xian}",

note = "Funding Information: This work was supported by the National Science Foundation of China (NSFC). We appreciate Professor J.H.R. K{\"a}gi from Department of Biochemistry of the University of Z{\"u}rich for his encouragement and fruitful discussion. ",

year = "2008",

month = nov,

doi = "10.1016/j.jinorgbio.2008.07.007",

language = "English (US)",

volume = "102",

pages = "1965--1972",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

number = "11",

}

TY - JOUR

T1 - Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)

AU - Ding, Zhi-Chun

AU - Zheng, Qi

AU - Cai, Bin

AU - Ni, Feng Yun

AU - Yu, Wen Hao

AU - Teng, Xing Chen

AU - Gao, Yuan

AU - Liu, Fang

AU - Chen, Dong

AU - Wang, Yang

AU - Wu, Hou Ming

AU - Sun, Hong Zhe

AU - Zhang, Ming Jie

AU - Tan, Xiang Shi

AU - Huang, Zhong Xian

N1 - Funding Information: This work was supported by the National Science Foundation of China (NSFC). We appreciate Professor J.H.R. Kägi from Department of Biochemistry of the University of Zürich for his encouragement and fruitful discussion.

PY - 2008/11

Y1 - 2008/11

N2 - Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the 6CPCP9 motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.

AB - Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the 6CPCP9 motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.

KW - Cell bioassay

KW - Metallothionein (MT)

KW - Mutation

KW - Neuronal growth inhibitory factor (GIF)

KW - Structure-function relationship

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DO - 10.1016/j.jinorgbio.2008.07.007

M3 - Short survey

C2 - 18757100

AN - SCOPUS:53249140316

SN - 0162-0134

VL - 102

SP - 1965

EP - 1972

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 11

ER -

Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)

Abstract

Keywords

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