Substantivity of chlorhexidine to human dentin

Marcela R. Carrilho, Ricardo M. Carvalho, Ethan N. Sousa, José Nicolau, Lorenzo Breschi, Annalisa Mazzoni, Leo Tjäderhane, Franklin R. Tay, Kelli Agee, David H. Pashley

Research output: Contribution to journalArticlepeer-review

164 Scopus citations


Objectives: To better comprehend the role of CHX in the preservation of resin-dentin bonds, this study investigated the substantivity of CHX to human dentin. Material and methods: Dentin disks (n = 45) were obtained from the mid-coronal portion of human third molars. One-third of dentin disks were kept mineralized (MD), while the other two-thirds had one of the surfaces partially demineralized with 37% phosphoric acid for 15 s (PDD) or they were totally demineralized with 10% phosphoric acid (TDD). Disks of hydroxyapatite (HA) were also prepared. Specimens were treated with: (1) 10 μL of distilled water (controls), (2) 10 μL of 0.2% chlorhexidine diacetate (0.2% CHX) or (3) 10 μL of 2% chlorhexidine diacetate (2% CHX). Then, they were incubated in 1 mL of PBS (pH 7.4, 37 °C). Substantivity was evaluated as a function of the CHX-applied dose after: 0.5 h, 1 h, 3 h, 6 h, 24 h, 168 h (1 week), 672 h (4 weeks) and 1344 h (8 weeks) of incubation. CHX concentration in eluates was spectrophotometrically analyzed at 260 nm. Results: Significant amounts of CHX remained retained in dentin substrates (MD, PPD or TDD), independent on the CHX-applied dose or time of incubation (p < 0.05). High amounts of retained CHX onto HA were observed only for specimens treated with the highest concentration of CHX (2%) (p < 0.05). Conclusion: The outstanding substantivity of CHX to dentin and its reported effect on the inhibition of dentinal proteases may explain why CHX can prolong the durability of resin-dentin bonds.

Original languageEnglish (US)
Pages (from-to)779-785
Number of pages7
JournalDental Materials
Issue number8
StatePublished - Aug 2010


  • Binding
  • Chlorhexidine
  • Dentin
  • Substantivity

ASJC Scopus subject areas

  • General Materials Science
  • General Dentistry
  • Mechanics of Materials


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