TY - JOUR
T1 - Sulforaphane suppresses angiogenesis and disrupts endothelial mitotic progression and microtubule polymerization
AU - Jackson, Steven J.T.
AU - Singletary, Keith W.
AU - Venema, Richard C.
N1 - Funding Information:
This work was supported by grant number 05A079 from the American Institute for Cancer Research, and by HL72768 from the National Institute of Health.
PY - 2007/2
Y1 - 2007/2
N2 - Sulforaphane (SUL), an isothiocyanate derived from broccoli and other cruciferous vegetables, is known to induce phase II detoxification enzymes, disrupt cancer cell microtubule polymerization, and trigger cell cycle arrest in breast and colon cancer cells. Here, we provide the first evidence that SUL also acts to inhibit angiogenesis via suppression of endothelial cellproliferation. Bovine aortic endothelial (BAE) cells were exposed to concentrations of up to 15 μM SUL prior to cell cycle analysis and mitotic index quantification. Within 24 h, 15 μM SUL clearly induced G2/M accumulation and pre-metaphase arrest in BAE cells. Moreover, immunofluorescence tubulin staining indicated that this same SUL concentration was efficacious in not only disrupting mitotic progression, but also in perturbing normal polymerization of mitotic (and cytoplasmic) microtubules. Furthermore, daily administration of SUL (100 nmol/day, i.v. for 7 days) to female Balb/c mice bearing VEGF-impregnated Matrigel plugs strongly and significantly (P < 0.05) suppressed angiogenesis progression as measured by hemoglobin concentration. Taken together, these findings suggest that the endothelial cell population is a novel target of SUL action both in vitro and in vivo. This mechanism of SUL-induced endothelial microtubule disruption and early mitotic arrest may further discern a potential role of SUL as a chemopreventive agent.
AB - Sulforaphane (SUL), an isothiocyanate derived from broccoli and other cruciferous vegetables, is known to induce phase II detoxification enzymes, disrupt cancer cell microtubule polymerization, and trigger cell cycle arrest in breast and colon cancer cells. Here, we provide the first evidence that SUL also acts to inhibit angiogenesis via suppression of endothelial cellproliferation. Bovine aortic endothelial (BAE) cells were exposed to concentrations of up to 15 μM SUL prior to cell cycle analysis and mitotic index quantification. Within 24 h, 15 μM SUL clearly induced G2/M accumulation and pre-metaphase arrest in BAE cells. Moreover, immunofluorescence tubulin staining indicated that this same SUL concentration was efficacious in not only disrupting mitotic progression, but also in perturbing normal polymerization of mitotic (and cytoplasmic) microtubules. Furthermore, daily administration of SUL (100 nmol/day, i.v. for 7 days) to female Balb/c mice bearing VEGF-impregnated Matrigel plugs strongly and significantly (P < 0.05) suppressed angiogenesis progression as measured by hemoglobin concentration. Taken together, these findings suggest that the endothelial cell population is a novel target of SUL action both in vitro and in vivo. This mechanism of SUL-induced endothelial microtubule disruption and early mitotic arrest may further discern a potential role of SUL as a chemopreventive agent.
KW - Angiogenesis
KW - Microtubules
KW - Mitotic arrest
KW - Sulforaphane
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U2 - 10.1016/j.vph.2006.06.015
DO - 10.1016/j.vph.2006.06.015
M3 - Article
C2 - 16938492
AN - SCOPUS:33845593776
SN - 1537-1891
VL - 46
SP - 77
EP - 84
JO - Vascular Pharmacology
JF - Vascular Pharmacology
IS - 2
ER -