Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene

Mustafa Culha; David Stokes; Tuan Vo-Dinh

doi:10.1586/14737159.3.5.669

Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene

Mustafa Culha, David Stokes, Tuan Vo-Dinh

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH₂)₆-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

Original language	English (US)
Pages (from-to)	669-675
Number of pages	7
Journal	Expert Review of Molecular Diagnostics
Volume	3
Issue number	5
DOIs	https://doi.org/10.1586/14737159.3.5.669
State	Published - Sep 2003
Externally published	Yes

Keywords

BCL2
Cancer
Detection
Immobilization
Rhodamine-B
SAM
SERS

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Medicine
Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1586/14737159.3.5.669

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title = "Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene",

abstract = "A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.",

keywords = "BCL2, Cancer, Detection, Immobilization, Rhodamine-B, SAM, SERS",

author = "Mustafa Culha and David Stokes and Tuan Vo-Dinh",

note = "Funding Information: This work was sponsored by the US Department of Energy (DOE) Office of Biological and Environmental Research and the DOE Chemical and Biological Nonproliferation Program and under contract DE-AC05-00OR22725 with UT-Battelle, LLC. Mustafa Culha and David Stokes are supported by an appointment to Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.",

year = "2003",

month = sep,

doi = "10.1586/14737159.3.5.669",

language = "English (US)",

volume = "3",

pages = "669--675",

journal = "Expert Review of Molecular Diagnostics",

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T1 - Surface-enhanced Raman scattering for cancer diagnostics

T2 - Detection of the BCL2 gene

AU - Culha, Mustafa

AU - Stokes, David

AU - Vo-Dinh, Tuan

N1 - Funding Information: This work was sponsored by the US Department of Energy (DOE) Office of Biological and Environmental Research and the DOE Chemical and Biological Nonproliferation Program and under contract DE-AC05-00OR22725 with UT-Battelle, LLC. Mustafa Culha and David Stokes are supported by an appointment to Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.

PY - 2003/9

Y1 - 2003/9

N2 - A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

AB - A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

KW - BCL2

KW - Cancer

KW - Detection

KW - Immobilization

KW - Rhodamine-B

KW - SAM

KW - SERS

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Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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