Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene

Mustafa Culha, David Stokes, Tuan Vo-Dinh

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

Original languageEnglish (US)
Pages (from-to)669-675
Number of pages7
JournalExpert Review of Molecular Diagnostics
Issue number5
StatePublished - Sep 2003
Externally publishedYes


  • BCL2
  • Cancer
  • Detection
  • Immobilization
  • Rhodamine-B
  • SAM
  • SERS

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Medicine
  • Molecular Biology
  • Genetics


Dive into the research topics of 'Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene'. Together they form a unique fingerprint.

Cite this