Surface-enhanced Raman scattering from living cells: From differentiating healthy and cancerous cell to cytotoxicity assessment

Gamze Kuku, Melike Sariçam, Sevda Mert, Mustafa Çulha

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


There is an ongoing effort to obtain molecular level information from living cells using surface-enhanced Raman scattering (SERS) not only to understand changes of cellular processes upon exposure to external stimuli but also to decide the status of cells; whether they are healthy or abnormal. In our research effort, we investigate how much information can be obtained from living cells to use for decision making about the cellular processes using SERS. The undertaken studies include cytotoxicity assessment of the nanomaterials and differentiation of the healthy and cancer cells. In the first case, A549 (lung cancer) and HDF (human dermal fibroblast) cells were incubated with 50 nm gold nanoparticles (AuNP) and exposed to three different nanoparticles (Zinc oxide nanoparticles (ZnO NPs), titanium dioxide nanoparticles (TiO2) and single walled carbon nanotubes (SWCNTs)) to perform SERS analysis and track the cellular response to these nanomaterials (NMs). After the principal component analysis on the spectral data, it was shown that the NPs exposed samples could be differentiated through SERS. In the second case, SERS spectra obtained from human kidney adenocarcinoma (ACHN), human kidney carcinoma (A-498) and non-cancerous human kidney embryonic cells (HEK 293) were used to diagnose metastatic, primary and non-cancerous cell lines. Linear discriminant analysis (LDA) based on principal component analysis (PCA) was applied to collected multidimensional SERS spectral data set to differentiate three different cell lines.

Original languageEnglish (US)
Title of host publicationSmart Biomedical and Physiological Sensor Technology XII
EditorsBrian M. Cullum, Eric S. McLamore
ISBN (Electronic)9781628416039
StatePublished - 2015
Externally publishedYes
EventSmart Biomedical and Physiological Sensor Technology XII - Baltimore, United States
Duration: Apr 23 2015Apr 24 2015

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceSmart Biomedical and Physiological Sensor Technology XII
Country/TerritoryUnited States


  • SERS
  • chemometrics
  • differentiation
  • nanotoxicity
  • renal cell
  • single cell analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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