Surface-Enhanced Raman Scattering Substrate Based on a Self-Assembled Monolayer for Use in Gene Diagnostics

Mustafa Culha; David Stokes; Leonardo R. Allain; Tuan Vo-Dinh

doi:10.1021/ac0346003

Surface-Enhanced Raman Scattering Substrate Based on a Self-Assembled Monolayer for Use in Gene Diagnostics

Mustafa Culha, David Stokes, Leonardo R. Allain, Tuan Vo-Dinh

Research output: Contribution to journal › Article › peer-review

172 Scopus citations

Abstract

The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH₂) ₆-SsDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microcopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.

Original language	English (US)
Pages (from-to)	6196-6201
Number of pages	6
Journal	Analytical Chemistry
Volume	75
Issue number	22
DOIs	https://doi.org/10.1021/ac0346003
State	Published - Nov 15 2003
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ac0346003

Cite this

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title = "Surface-Enhanced Raman Scattering Substrate Based on a Self-Assembled Monolayer for Use in Gene Diagnostics",

abstract = "The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH2) 6-SsDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microcopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.",

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AU - Culha, Mustafa

AU - Stokes, David

AU - Allain, Leonardo R.

AU - Vo-Dinh, Tuan

PY - 2003/11/15

Y1 - 2003/11/15

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AB - The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH-(CH2) 6-SsDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microcopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.

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Surface-Enhanced Raman Scattering Substrate Based on a Self-Assembled Monolayer for Use in Gene Diagnostics

Abstract

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