TY - JOUR
T1 - Surface-Enhanced Raman Scattering to Evaluate Nanomaterial Cytotoxicity on Living Cells
AU - Kuku, Gamze
AU - Saricam, Melike
AU - Akhatova, Farida
AU - Danilushkina, Anna
AU - Fakhrullin, Rawil
AU - Culha, Mustafa
N1 - Funding Information:
The authors acknowledges the financial support of The Scientific and Technological Council of Turkey (TUBITAK) (Project No. 113Z554) and Yeditepe University. The authors also acknowledge the financial support through the COST action, NanoSpectroscopy, Grant MP1302. Nanoparticle imaging experiments (TEM and EDF) were supported by the Russian Science Foundation Grant No. 14-14-00924.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/10/4
Y1 - 2016/10/4
N2 - The increasing number of reports about false positive or negative results from conventional cytotoxicity assays of nanomaterials (NMs) suggests that more reliable NM toxicity assessment methods should be developed. Here, we report a novel approach for nanotoxicity evaluation based on surface-enhanced Raman spectroscopy (SERS). Three model NMs were tested on two model cell lines and the results were validated by WST-1 cytotoxicity assay and annexin V-FITC/propidium iodide (PI) staining as apoptosis-necrosis assay. The localization of nanoparticles (NPs) in the cells and the cellular conditions upon NP incubation were visualized by transmission electron microscopy (TEM) and enhanced dark-field (EDF) microscopy. SERS revealed a broader view on the consequences of cell-NM interactions compared to the conventional cytotoxicity assays where only one aspect of toxicity can be measured by one assay type. The results suggest that SERS can significantly contribute to the cytotoxicity evaluation bypassing NM or assay component-related complications with less effort.
AB - The increasing number of reports about false positive or negative results from conventional cytotoxicity assays of nanomaterials (NMs) suggests that more reliable NM toxicity assessment methods should be developed. Here, we report a novel approach for nanotoxicity evaluation based on surface-enhanced Raman spectroscopy (SERS). Three model NMs were tested on two model cell lines and the results were validated by WST-1 cytotoxicity assay and annexin V-FITC/propidium iodide (PI) staining as apoptosis-necrosis assay. The localization of nanoparticles (NPs) in the cells and the cellular conditions upon NP incubation were visualized by transmission electron microscopy (TEM) and enhanced dark-field (EDF) microscopy. SERS revealed a broader view on the consequences of cell-NM interactions compared to the conventional cytotoxicity assays where only one aspect of toxicity can be measured by one assay type. The results suggest that SERS can significantly contribute to the cytotoxicity evaluation bypassing NM or assay component-related complications with less effort.
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U2 - 10.1021/acs.analchem.6b02917
DO - 10.1021/acs.analchem.6b02917
M3 - Article
C2 - 27611981
AN - SCOPUS:84989834034
SN - 0003-2700
VL - 88
SP - 9813
EP - 9820
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -