TY - JOUR
T1 - Synapsins in the vertebrate retina
T2 - Absence from ribbon synapses and heterogeneous distribution among conventional synapses
AU - Mandell, James W.
AU - Townes-Anderson, Ellen
AU - Czernik, Andrew J.
AU - Cameron, Richard
AU - Greengard, Paul
AU - De Camilli, Pietro
PY - 1990/7
Y1 - 1990/7
N2 - The vertebrate retina contains two ultrastructurally distinct types of vesicle-containing synapses: conventional synapses, made predominantly by amacrine cells, and ribbon synapses, formed by photoreceptor and bipolar cells. To identify molecular differences between these synapse types, we have compared the distribution of the synapsins, a family of nerve terminal phosphoproteins, with that of synaptophysin (p38) and SV2, two intrinsic membrane proteins of synaptic vesicles. We report an absence of synapsin I and II immunoreactivity from all ribbon-containing nerve terminals. These include terminals of rod cells in developing and adult rat retina, rod and cone cells in monkey and salamander retinas, and rat bipolar cells. Furthermore, we show that synapsins I and II are differentially distributed among conventional synapses of amacrine cells. The absence of the synapsins from ribbon synapses suggests that vesicle clustering and mobilization in these terminals differ from that in conventional synapses.
AB - The vertebrate retina contains two ultrastructurally distinct types of vesicle-containing synapses: conventional synapses, made predominantly by amacrine cells, and ribbon synapses, formed by photoreceptor and bipolar cells. To identify molecular differences between these synapse types, we have compared the distribution of the synapsins, a family of nerve terminal phosphoproteins, with that of synaptophysin (p38) and SV2, two intrinsic membrane proteins of synaptic vesicles. We report an absence of synapsin I and II immunoreactivity from all ribbon-containing nerve terminals. These include terminals of rod cells in developing and adult rat retina, rod and cone cells in monkey and salamander retinas, and rat bipolar cells. Furthermore, we show that synapsins I and II are differentially distributed among conventional synapses of amacrine cells. The absence of the synapsins from ribbon synapses suggests that vesicle clustering and mobilization in these terminals differ from that in conventional synapses.
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U2 - 10.1016/0896-6273(90)90030-J
DO - 10.1016/0896-6273(90)90030-J
M3 - Article
C2 - 2114884
AN - SCOPUS:0025345021
SN - 0896-6273
VL - 5
SP - 19
EP - 33
JO - Neuron
JF - Neuron
IS - 1
ER -