Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions

B. A. Wallace; Kunihiko Gekko; Søren Vrønning Hoffmann; Yi Hung Lin; John C. Sutherland; Ye Tao; Frank Wien; Robert W. Janes

doi:10.1016/j.nima.2010.10.135

Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions

B. A. Wallace
, Kunihiko Gekko
, Søren Vrønning Hoffmann
, Yi Hung Lin
, John C. Sutherland
, Ye Tao
, Frank Wien
, Robert W. Janes

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.

Original language	English (US)
Pages (from-to)	177-178
Number of pages	2
Journal	Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume	649
Issue number	1
DOIs	https://doi.org/10.1016/j.nima.2010.10.135
State	Published - Sep 1 2011
Externally published	Yes

Keywords

Conformational changes
Macromolecular interactions
Protein secondary structure
Synchrotron radiation circular dichroism spectroscopy

ASJC Scopus subject areas

Nuclear and High Energy Physics
Instrumentation

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10.1016/j.nima.2010.10.135

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Wallace, B. A., Gekko, K., Vrønning Hoffmann, S., Lin, Y. H., Sutherland, J. C., Tao, Y., Wien, F., & Janes, R. W. (2011). Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions. Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 649(1), 177-178. https://doi.org/10.1016/j.nima.2010.10.135

Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions. / Wallace, B. A.; Gekko, Kunihiko; Vrønning Hoffmann, Søren et al.
In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 649, No. 1, 01.09.2011, p. 177-178.

Research output: Contribution to journal › Article › peer-review

Wallace, BA, Gekko, K, Vrønning Hoffmann, S, Lin, YH, Sutherland, JC, Tao, Y, Wien, F & Janes, RW 2011, 'Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions', Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, vol. 649, no. 1, pp. 177-178. https://doi.org/10.1016/j.nima.2010.10.135

Wallace BA, Gekko K, Vrønning Hoffmann S, Lin YH, Sutherland JC, Tao Y et al. Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions. Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 2011 Sep 1;649(1):177-178. doi: 10.1016/j.nima.2010.10.135

Wallace, B. A. ; Gekko, Kunihiko ; Vrønning Hoffmann, Søren et al. / Synchrotron radiation circular dichroism (SRCD) spectroscopy : An emerging method in structural biology for examining protein conformations and protein interactions. In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 2011 ; Vol. 649, No. 1. pp. 177-178.

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abstract = "Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.",

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AU - Wien, Frank

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Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions

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