TY - JOUR
T1 - Synergistic effect of selenium and melatonin on neuroprotection in cerebral ischemia in rats
AU - Ahmad, Ajmal
AU - Khan, Mohd Moshahid
AU - Ishrat, Tauheed
AU - Khan, M. Badruzzaman
AU - Khuwaja, Gulrana
AU - Raza, Syed Shadab
AU - Shrivastava, Pallavi
AU - Islam, Fakhrul
N1 - Funding Information:
Acknowledgments Authors are thankful to the Department of Ayurveda, Yoga and Naturalpathy, Unani, Siddha and Homeopath (AYUSH), Ministry of Health and Family Welfare, Government of India, New Delhi for financial assistance. The authors wish to thank Mr. Dharamvir Singh for his assistance.
PY - 2011/1
Y1 - 2011/1
N2 - The synergistic scavenger effects of selenium and melatonin collectively we called Se-Mel was studied on the prevention of neuronal injury induced by ischemia/reperfusion. Male Wistar rats were treated with sodium selenite (0.1 mg/kg, i.p.) and melatonin (10 mg/kg, i.p.) 30 min before the middle carotid artery occlusion (MCAO) and immediately after MCAO to male Wistar rats and was continued for 3 days once daily at the interval of 24 h. Behavioral activity (spontaneous motor activity and motor deficit) was improved in Se-Mel-treated rats as compared to MCAO group rats. The level of glutathione and the activity of antioxidant enzymes was depleted significantly while the content of thiobarbituric acid reactive substances, protein carbonyl, and nitric oxide radical (NO•) was increased significantly in MCAO group. Systemic administration of Se-Mel ameliorated oxidative stress and improves ischemia/reperfusion-induced focal cerebral ischemia. Se-Mel also inhibited inducible nitric oxide synthase expression in Se-Mel+MCAO group as compared to MCAO group rats. Thus, Se-Mel has shown an excellent neuroprotective effect against ischemia/reperfusion injury through an anti-ischemic pathway. In conclusion, we demonstrated that the pretreatment with Se-Mel at the onset of reperfusion, reduced post-ischemic damage, and improved neurological outcome following transient focal cerebral ischemia in male Wistar rat.
AB - The synergistic scavenger effects of selenium and melatonin collectively we called Se-Mel was studied on the prevention of neuronal injury induced by ischemia/reperfusion. Male Wistar rats were treated with sodium selenite (0.1 mg/kg, i.p.) and melatonin (10 mg/kg, i.p.) 30 min before the middle carotid artery occlusion (MCAO) and immediately after MCAO to male Wistar rats and was continued for 3 days once daily at the interval of 24 h. Behavioral activity (spontaneous motor activity and motor deficit) was improved in Se-Mel-treated rats as compared to MCAO group rats. The level of glutathione and the activity of antioxidant enzymes was depleted significantly while the content of thiobarbituric acid reactive substances, protein carbonyl, and nitric oxide radical (NO•) was increased significantly in MCAO group. Systemic administration of Se-Mel ameliorated oxidative stress and improves ischemia/reperfusion-induced focal cerebral ischemia. Se-Mel also inhibited inducible nitric oxide synthase expression in Se-Mel+MCAO group as compared to MCAO group rats. Thus, Se-Mel has shown an excellent neuroprotective effect against ischemia/reperfusion injury through an anti-ischemic pathway. In conclusion, we demonstrated that the pretreatment with Se-Mel at the onset of reperfusion, reduced post-ischemic damage, and improved neurological outcome following transient focal cerebral ischemia in male Wistar rat.
KW - Middle carotid artery occlusion
KW - Neurobehavior
KW - Neuroprotection
KW - Oxidative stress
KW - Se-Mel
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U2 - 10.1007/s12011-010-8643-z
DO - 10.1007/s12011-010-8643-z
M3 - Article
C2 - 20358308
AN - SCOPUS:78651243694
SN - 0163-4984
VL - 139
SP - 81
EP - 96
JO - Biological Trace Element Research
JF - Biological Trace Element Research
IS - 1
ER -