Temporal endothelin dynamics of the myocardial interstitium and systemic circulation in cardiopulmonary bypass

C. Allyson Walker, Simona C. Baicu, Aron T. Goldberg, Colin E. Widener, David J. Fary, Daniel K. Almany, Adviye Ergul, Fred A. Crawford, Francis G. Spinale

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Objective: Increased systemic levels of the bioactive peptide endothelin 1 during and after cardioplegic arrest and cardiopulmonary bypass have been well documented. However, endothelin 1 is synthesized locally, and therefore myocardial endothelin 1 production during and after cardiopulmonary bypass remains unknown. Methods: Pigs (n = 11) were instrumented for cardiopulmonary bypass, and cardioplegic arrest was initiated. Myocardial interstitial and systemic arterial levels of endothelin 1 were measured before cardiopulmonary bypass, throughout bypass and cardioplegic arrest (90 minutes), and up to 90 minutes after separation from bypass. Myocardial interstitial endothelin 1 was determined by microdialysis and radioimmunoassay. Results: Baseline myocardial endothelin 1 levels were higher than systemic endothelin 1 levels (25.6 ± 6.7 vs 8.3 ± 1.1 fmol/mL, P < .05). With the onset of bypass, myocardial endothelin 1 increased by 327% ± 92% from baseline (P < .05), which preceded the increase in systemic endothelin 1 levels. Conclusion: Myocardial compartmentalization of endothelin 1 exists in vivo. Cardiopulmonary bypass and cardioplegic arrest induce temporal differences in endothelin 1 levels within the myocardial interstitium and systemic circulation, which, in turn, may influence left ventricular function in the postbypass period.

Original languageEnglish (US)
Pages (from-to)864-871
Number of pages8
JournalJournal of Thoracic and Cardiovascular Surgery
Issue number5
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine


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