TY - JOUR
T1 - Temporal regulation of equine herpesvirus type 3 transcription
AU - Sullivan, Donna C.
AU - Gray, Wayne L.
AU - Caughman, Gretchen B
AU - Robertson, Alice T.
AU - O'Callaghan, Dennis J.
N1 - Funding Information:
We thank Cynthia S. Harrison for typing the manuscript and Suzanne Zavecz for expert technical assistance. Support for this investigation was obtained from Public Health Service research grants AI 22001 and AI 22894 from the National Institutes of Health, a Grayson Foundation Inc., research grant, a grant from the Louisiana State University System Biotechnology Institute, and grant 86-CRCR-2257 from the U.S. Department of Agriculture Biotechnology Program.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990/2
Y1 - 1990/2
N2 - The transcription of equine herpesvirus type 3 (EHV-3; equine coital exanthema virus) has been examined and found to be temporally regulated into three classes: immediate early (IE), early (E), and late (L). Hybridization of in vivo 32PO4-labeled transcripts revealed that IE transcript(s) are derived exclusively from the inverted repeat segments (IRs) of the viral genome, while E and L transcripts are not restricted to any specific region of the genome. Northern blot analysis of EHV-3 IE RNA revealed a single transcript of approximately 5.7 kb (3.8 MDa). We have previously shown that transcription of equine herpesvirus type 1 (EHV-1) DNA is temporally regulated and produces a single 6 kb IE RNA which is derived from the IRs segments. In this paper, we show that the EHV-1 and EHV-3 IE RNA species are homologous, reflecting the colinearity of the genomes of these two related viruses. While four IE polypeptides are synthesized in EHV-1 infected cells in the presence of actinomycin D following the removal of a cycloheximide block, only one major IE polypeptide (180 kDa) is detectable in EHV-3 infected cells under these conditions. However, immunoprecipitation of EHV-3 infected cell extracts with polyvalent rabbit antisera to IE1 of EHV-1 revealed at least two other viral specific IE polypeptides.
AB - The transcription of equine herpesvirus type 3 (EHV-3; equine coital exanthema virus) has been examined and found to be temporally regulated into three classes: immediate early (IE), early (E), and late (L). Hybridization of in vivo 32PO4-labeled transcripts revealed that IE transcript(s) are derived exclusively from the inverted repeat segments (IRs) of the viral genome, while E and L transcripts are not restricted to any specific region of the genome. Northern blot analysis of EHV-3 IE RNA revealed a single transcript of approximately 5.7 kb (3.8 MDa). We have previously shown that transcription of equine herpesvirus type 1 (EHV-1) DNA is temporally regulated and produces a single 6 kb IE RNA which is derived from the IRs segments. In this paper, we show that the EHV-1 and EHV-3 IE RNA species are homologous, reflecting the colinearity of the genomes of these two related viruses. While four IE polypeptides are synthesized in EHV-1 infected cells in the presence of actinomycin D following the removal of a cycloheximide block, only one major IE polypeptide (180 kDa) is detectable in EHV-3 infected cells under these conditions. However, immunoprecipitation of EHV-3 infected cell extracts with polyvalent rabbit antisera to IE1 of EHV-1 revealed at least two other viral specific IE polypeptides.
KW - EHV-3 transcription
KW - Equine herpesvirus type 3
KW - Immediate early gene
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U2 - 10.1016/0168-1702(90)90004-U
DO - 10.1016/0168-1702(90)90004-U
M3 - Article
C2 - 2157315
AN - SCOPUS:0025012494
SN - 0168-1702
VL - 15
SP - 135
EP - 148
JO - Virus Research
JF - Virus Research
IS - 2
ER -