The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations

Nicolai S.C. Van Oers, Brett Tohlen, Bernard Malissen, Carolyn R. Moomaw, Steve Afendis, Clive A. Slaughter

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


The T cell receptor (TCR) ζ subunit contains three immunoreceptor tyrosine-based activation motifs (ITAMs) that translate effective extracellular ligand binding into intracellular signals by becoming phosphorylated into 21- and 23-kD forms. We report here that the 21-kD form of TCRζ is generated by phosphorylation of the tyrosines in the second and third ITAMs, whereas the 23-kD form is formed by the additional phosphorylation of the membrane-proximal ITAM tyrosines. The stable formation of the 21- and 23-kD species requires the binding of the tandem SH2 domains of ZAP-70. We also report that TCR-mediated signaling processes can proceed independently of either the 21- or 23-kD species of TCRζ.

Original languageEnglish (US)
Pages (from-to)322-328
Number of pages7
JournalNature Immunology
Issue number4
StatePublished - Oct 2000
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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