Abstract
The effects of high K+ exposure on the accumulation, of diacylglycerol (DAG) and on the activation of protein kinase C (PKC) have been studied in frog skeletal muscle. The endogenous DAG increased by treatment with 80mM K+ for different lengths of time. Correspondingly, high K+ exposure could induce a translocation of PKC. For instance, the membrane-associatad PKC activity (pmol/min·g muscle) was increased from the control value of 523±87 to 725±33 and 858±59 after 80mM K+ exposure for 2 and 60 minutes, while the cytosolic PKC activity was reduced from 548±35 to 307±20 and 254±46, respectively. The accumulation of DAG and the activation of PKC induced by high K+ exposure suggest a possible role of the pathway of DAG/PKC in the regulation of the excitation-contraction coupling in skeletal muscle fibres.
Original language | English (US) |
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Pages (from-to) | 574-575 |
Number of pages | 2 |
Journal | Acta Biochimica et Biophysica Sinica |
Volume | 28 |
Issue number | 5 |
State | Published - Dec 1 1996 |
Externally published | Yes |
Keywords
- Cellular signaling transduction
- Diacylglycerol
- Excitation-contraction coupling
- Protein kinase C
ASJC Scopus subject areas
- Biochemistry
- Biophysics