The antioxidant requirement for plasma membrane repair in skeletal muscle

Mohamed Labazi, Anna K. McNeil, Timothy Kurtz, Taylor C. Lee, Ronald B. Pegg, José Pedro Friedmann Angeli, Marcus Conrad, Paul L McNeil

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Vitamin E (VE) deficiency results in pronounced muscle weakness and atrophy but the cell biological mechanism of the pathology is unknown. We previously showed that VE supplementation promotes membrane repair in cultured cells and that oxidants potently inhibit repair. Here we provide three independent lines of evidence that VE is required for skeletal muscle myocyte plasma membrane repair in vivo. We also show that when another lipid-directed antioxidant, glutathione peroxidase 4 (Gpx4), is genetically deleted in mouse embryonic fibroblasts, repair fails catastrophically, unless cells are supplemented with VE. We conclude that lipid-directed antioxidant activity provided by VE, and possibly also Gpx4, is an essential component of the membrane repair mechanism in skeletal muscle. This work explains why VE is essential to muscle health and identifies VE as a requisite component of the plasma membrane repair mechanism in vivo.

Original languageEnglish (US)
Pages (from-to)246-253
Number of pages8
JournalFree Radical Biology and Medicine
StatePublished - May 10 2015


  • Antioxidants
  • Free radicals
  • Membrane repair
  • Skeletal muscle
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


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