TY - JOUR
T1 - The circadian expression of osteogenic factors in periodontal tissue loading mechanical force
T2 - new concepts of the personalized orthodontic care
AU - Qin, Xu
AU - Li, Qilin
AU - Chen, Weimin
AU - Bai, Yumin
AU - Baban, Babak
AU - Mao, Jing
N1 - Funding Information:
Funding The study was funded by the National Science Foundation of China (No. 81170986 and No.81800891).
Publisher Copyright:
© 2019, European Association for Predictive, Preventive and Personalised Medicine (EPMA).
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Objective: The need for orthodontic treatment continues to increase. Strategies that shorten the treatment course and reduce discomfort are most welcome in clinic. Circadian rhythm plays important role in various physiological processes, including bone formation. This study intended to depict a possible circadian releasing property of the osteogenic factors within the periodontal tissue during orthodontic treatment, which may direct a more efficient and satisfactory orthodontic treatment to the patient. Methods: Primary periodontal ligament cells (PDLCs) were obtained from the Sprague-Dawley (SD) rats. An equibiaxial strain value of 12% was applied on rat PDLCs (rPDLCs). After 2 h stimuli of 10−7 M dexamethasone (DX), the osteogenic genes’ expressions were detected by real-time polymerase chain reaction (RT-PCR) at Zeitgeber times 0, 4, 8, 12, 16, 20, and 24. An orthodontic appliance was placed on 45 SD rats. Animals were maintained under 12-h light/dark periods and euthanized at 9 time points over the diurnal cycle. The orthodontic sensitive tissues of the mesial root of the maxillary first molar were collected for RT-PCR and immunohistological assay. Results: The rPDLCs displayed typical fibroblastic spindle shape, and subcultured steadily in vitro. Induced by DX, the mRNA expression of Col-1, OPN, and IBSP within the loaded/unloaded rPDLCs oscillated as that of the main clock gene Per-1. The osteogenic genes’ expressions as well as the protein releases sustained a circadian oscillation trend in vivo. Conclusions: This study indicates the existence of a circadian rhythm of the osteogenic factors within the orthodontic sensitive tissues, which highlights the importance of precise timing of force loading in further orthodontic treatment. Thus, a periodicity pattern of orthodontic traction at night may prove a more efficient tooth movement while minimizing the treatment window and discomfort complains.
AB - Objective: The need for orthodontic treatment continues to increase. Strategies that shorten the treatment course and reduce discomfort are most welcome in clinic. Circadian rhythm plays important role in various physiological processes, including bone formation. This study intended to depict a possible circadian releasing property of the osteogenic factors within the periodontal tissue during orthodontic treatment, which may direct a more efficient and satisfactory orthodontic treatment to the patient. Methods: Primary periodontal ligament cells (PDLCs) were obtained from the Sprague-Dawley (SD) rats. An equibiaxial strain value of 12% was applied on rat PDLCs (rPDLCs). After 2 h stimuli of 10−7 M dexamethasone (DX), the osteogenic genes’ expressions were detected by real-time polymerase chain reaction (RT-PCR) at Zeitgeber times 0, 4, 8, 12, 16, 20, and 24. An orthodontic appliance was placed on 45 SD rats. Animals were maintained under 12-h light/dark periods and euthanized at 9 time points over the diurnal cycle. The orthodontic sensitive tissues of the mesial root of the maxillary first molar were collected for RT-PCR and immunohistological assay. Results: The rPDLCs displayed typical fibroblastic spindle shape, and subcultured steadily in vitro. Induced by DX, the mRNA expression of Col-1, OPN, and IBSP within the loaded/unloaded rPDLCs oscillated as that of the main clock gene Per-1. The osteogenic genes’ expressions as well as the protein releases sustained a circadian oscillation trend in vivo. Conclusions: This study indicates the existence of a circadian rhythm of the osteogenic factors within the orthodontic sensitive tissues, which highlights the importance of precise timing of force loading in further orthodontic treatment. Thus, a periodicity pattern of orthodontic traction at night may prove a more efficient tooth movement while minimizing the treatment window and discomfort complains.
KW - Animal model
KW - Animal study
KW - Biomarker panel
KW - Circadian rhythm
KW - Expression pattern
KW - Mechanical force
KW - Orthodontic tooth movement
KW - Osteogenesis
KW - Personalized orthodonic care
KW - Predictive preventive personalised medicine
KW - Prognosis
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U2 - 10.1007/s13167-019-0161-2
DO - 10.1007/s13167-019-0161-2
M3 - Article
AN - SCOPUS:85063026941
SN - 1878-5077
VL - 10
SP - 13
EP - 20
JO - The EPMA Journal
JF - The EPMA Journal
IS - 1
ER -
