The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone

Lawrence C Layman; D. J. Roach; L. Plouffe; Paul G McDonough; J. T. Wilson

doi:10.1016/S0015-0282(16)53278-6

The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone

Lawrence C Layman, D. J. Roach, L. Plouffe, Paul G McDonough, J. T. Wilson

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSHβ has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSHβ-1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSHβ-1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSHβ gene with pFSHβ-1.4. RFLPs for FSHβ constitute the first step in the molecular analysis of disorders of FSH production.

Original language	English (US)
Pages (from-to)	261-265
Number of pages	5
Journal	Fertility and sterility
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/S0015-0282(16)53278-6
State	Published - 1990

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

Access to Document

10.1016/S0015-0282(16)53278-6

Cite this

@article{0120bc686fe54a988d6b060ebd537198,

title = "The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone",

abstract = "Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSHβ has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSHβ-1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSHβ-1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSHβ gene with pFSHβ-1.4. RFLPs for FSHβ constitute the first step in the molecular analysis of disorders of FSH production.",

author = "Layman, {Lawrence C} and Roach, {D. J.} and L. Plouffe and McDonough, {Paul G} and Wilson, {J. T.}",

year = "1990",

doi = "10.1016/S0015-0282(16)53278-6",

language = "English (US)",

volume = "53",

pages = "261--265",

journal = "Fertility and sterility",

issn = "0015-0282",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone

AU - Layman, Lawrence C

AU - Roach, D. J.

AU - Plouffe, L.

AU - McDonough, Paul G

AU - Wilson, J. T.

PY - 1990

Y1 - 1990

N2 - Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSHβ has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSHβ-1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSHβ-1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSHβ gene with pFSHβ-1.4. RFLPs for FSHβ constitute the first step in the molecular analysis of disorders of FSH production.

AB - Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSHβ has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSHβ-1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSHβ-1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSHβ gene with pFSHβ-1.4. RFLPs for FSHβ constitute the first step in the molecular analysis of disorders of FSH production.

UR - http://www.scopus.com/inward/record.url?scp=0025093451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025093451&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(16)53278-6

DO - 10.1016/S0015-0282(16)53278-6

M3 - Article

C2 - 1967584

AN - SCOPUS:0025093451

SN - 0015-0282

VL - 53

SP - 261

EP - 265

JO - Fertility and sterility

JF - Fertility and sterility

IS - 2

ER -

The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this