TY - JOUR
T1 - The effect of pH on the cytotoxicity of eluates from denture base resins
AU - Lefebvre, Carul A.
AU - Schuster, George S.
AU - Marr, John C.
AU - Knoernschild, Kent L.
PY - 1995
Y1 - 1995
N2 - This in uitro study examined the effects of environmental pH on eiution of potentially toxic substances from heat-, light-, and dual- (chemical plus light) polymerized denture base resins. Eluates were prepared by daily transfer of disks to fresh huffets at pH 4.0, 5,0, and 6,8 over a 5-day period. Oral epithelial cells were plated in culture dishes in medium containing the eluate. After 24 hours, cellular RNA synthesis was assessed by measuring tritiated uridine uptake. Effects of materials were compared to identical cultures that contained the appropriate buffer without the eluate. The results indicate that the cytotoxic components leach out of the denture base resins in different amounts and at different rates, and the amount of leaching can be affected by pH.
AB - This in uitro study examined the effects of environmental pH on eiution of potentially toxic substances from heat-, light-, and dual- (chemical plus light) polymerized denture base resins. Eluates were prepared by daily transfer of disks to fresh huffets at pH 4.0, 5,0, and 6,8 over a 5-day period. Oral epithelial cells were plated in culture dishes in medium containing the eluate. After 24 hours, cellular RNA synthesis was assessed by measuring tritiated uridine uptake. Effects of materials were compared to identical cultures that contained the appropriate buffer without the eluate. The results indicate that the cytotoxic components leach out of the denture base resins in different amounts and at different rates, and the amount of leaching can be affected by pH.
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M3 - Article
C2 - 7575962
AN - SCOPUS:0029264867
SN - 0893-2174
VL - 8
SP - 122
EP - 128
JO - International Journal of Prosthodontics
JF - International Journal of Prosthodontics
IS - 2
ER -