THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS

N. Fernandez; H. Festenstein; A. Alonzo; P. A. Biro; M. Labeta; E. Carolan; J. Houlihan; A. Mellor; R. Flavell

doi:10.1111/j.1744-313X.1986.tb01127.x

THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS

N. Fernandez, H. Festenstein, A. Alonzo, P. A. Biro, M. Labeta, E. Carolan, J. Houlihan, A. Mellor, R. Flavell

Infectious Disease

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Abstract

The cosmid H3.5, containing genes mapping to the murine H‐2 Qa region, was used to transfect L cells by the calcium phosphate co‐precipitation method. The resultant transfected cells expressed a Qa‐like determinant as detected by an immune serum raised against the transfectant cells and Qa specific monoclonal antibodies. Two‐dimensional gel analysis revealed the expression of a class I‐like heavy chain with a similar molecular mass to the Qa2 antigens of the positive strain B10 and B10.A but with a different isoelectric point. The cosmid H3.5 spans 40 kb of DNA and contains at least one complete Qa region gene which encodes the Qa‐like determinant detected in this study.

Original language	English (US)
Pages (from-to)	425-436
Number of pages	12
Journal	International Journal of Immunogenetics
Volume	13
Issue number	5-6
DOIs	https://doi.org/10.1111/j.1744-313X.1986.tb01127.x
State	Published - Oct 1986

ASJC Scopus subject areas

Immunology
Molecular Biology
Genetics
Genetics(clinical)

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10.1111/j.1744-313X.1986.tb01127.x

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abstract = "The cosmid H3.5, containing genes mapping to the murine H‐2 Qa region, was used to transfect L cells by the calcium phosphate co‐precipitation method. The resultant transfected cells expressed a Qa‐like determinant as detected by an immune serum raised against the transfectant cells and Qa specific monoclonal antibodies. Two‐dimensional gel analysis revealed the expression of a class I‐like heavy chain with a similar molecular mass to the Qa2 antigens of the positive strain B10 and B10.A but with a different isoelectric point. The cosmid H3.5 spans 40 kb of DNA and contains at least one complete Qa region gene which encodes the Qa‐like determinant detected in this study.",

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AU - Fernandez, N.

AU - Festenstein, H.

AU - Alonzo, A.

AU - Biro, P. A.

AU - Labeta, M.

AU - Carolan, E.

AU - Houlihan, J.

AU - Mellor, A.

AU - Flavell, R.

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THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS

Abstract

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