TY - JOUR
T1 - The in vitro perifused rat ovary
T2 - V. The significance of the follicle stimulating hormone and luteinizing hormone ratio on steroid release
AU - Soendoro, T.
AU - Diamond, M. P.
AU - Pepperell, J. R.
AU - Naftolin, F.
N1 - Funding Information:
This work was supported by NIH grant HD 22970.
PY - 1993
Y1 - 1993
N2 - In these studies, an in vitro perifusion model was used to compare stimulation of ovarian tissue with either human menopausal gonadotropin (hMG), which is an equal mixture of luteinizing hormone (LH) and follicle stimulating hormone (FSH), or with hMG plus added human FSH. Eight-hour perifusion studies were conducted on either whole, or dissected clusters of follicles from pregnant mare serum gonadotropin (PMSG)-treated rats. In the two groups, similar stimulatory protocols were used, consisting of a ramp stimulation over 60 min with either hMG (0-8 mlU/ml) or hMG plus FSH (0-8 mlU/ml hMG + 0-8 mlU/ml FSH), followed by hourly pulse stimulation with hMG (8-18 mlU/ml) or hMG plus FSH (8-18 mlU/ml hMG + 8-18 mlU/ml FSH), respectively. In the whole ovaries, no differences were detected in progesterone, testosterone, or estradiol secretion. However, in the cluster of follicles, an elevated hFSH/hMG ratio resulted in a significantly higher secretion of progesterone, testosterone and estradiol (n = 8; p < 0.05) than the steroids secreted by follicles perifused with hMG alone. In conclusion, an elevated FSH : LH ratio led to greater steroidogenic responses by the perifused cluster offollicles, but not by the whole ovary.
AB - In these studies, an in vitro perifusion model was used to compare stimulation of ovarian tissue with either human menopausal gonadotropin (hMG), which is an equal mixture of luteinizing hormone (LH) and follicle stimulating hormone (FSH), or with hMG plus added human FSH. Eight-hour perifusion studies were conducted on either whole, or dissected clusters of follicles from pregnant mare serum gonadotropin (PMSG)-treated rats. In the two groups, similar stimulatory protocols were used, consisting of a ramp stimulation over 60 min with either hMG (0-8 mlU/ml) or hMG plus FSH (0-8 mlU/ml hMG + 0-8 mlU/ml FSH), followed by hourly pulse stimulation with hMG (8-18 mlU/ml) or hMG plus FSH (8-18 mlU/ml hMG + 8-18 mlU/ml FSH), respectively. In the whole ovaries, no differences were detected in progesterone, testosterone, or estradiol secretion. However, in the cluster of follicles, an elevated hFSH/hMG ratio resulted in a significantly higher secretion of progesterone, testosterone and estradiol (n = 8; p < 0.05) than the steroids secreted by follicles perifused with hMG alone. In conclusion, an elevated FSH : LH ratio led to greater steroidogenic responses by the perifused cluster offollicles, but not by the whole ovary.
KW - In Vitro Perifusion
KW - Lh/Fsh Ratio
KW - Rat Ovaries
KW - Steroidogenesis
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U2 - 10.3109/09513599309152474
DO - 10.3109/09513599309152474
M3 - Article
C2 - 8506758
AN - SCOPUS:0027298347
SN - 0951-3590
VL - 7
SP - 13
EP - 17
JO - Gynecological Endocrinology
JF - Gynecological Endocrinology
IS - 1
ER -