The p38 and p34 polypeptides of growth cone particle membranes are the α- and β-subunits of G proteins

Brian T. Edmonds; Carolyn R. Moomaw; Joan T. Hsu; Clive A. Slaughter; Leland Ellis

doi:10.1016/0165-3806(90)90172-U

The p38 and p34 polypeptides of growth cone particle membranes are the α- and β-subunits of G proteins

Brian T. Edmonds, Carolyn R. Moomaw, Joan T. Hsu, Clive A. Slaughter, Leland Ellis

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Growth cone particle (GCP) membranes prepared from fetal day 17 rat brain are comprised of 5 major polypeptides as analyzed by SDS-PAGE: tubulin (p52), actin (p42), pp46/GAP-43 and two unidentified species, p38 and p34^14,46,52. Antibodies specific for the α- and β-subunits of G proteins recognize p38 and p34, respectively, on immunoblots following one- and two-dimensional electrophoretic separation. That G protein subunits comprise major species of GCP membrane-associated polypeptides suggests a role for G proteins in transmembrane signaling in nerve growth cones.

Original language	English (US)
Pages (from-to)	131-136
Number of pages	6
Journal	Developmental Brain Research
Volume	56
Issue number	1
DOIs	https://doi.org/10.1016/0165-3806(90)90172-U
State	Published - Oct 1 1990
Externally published	Yes

Keywords

G protein
Growth cone
Growth cone particle
Growth cone particle membrane

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

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10.1016/0165-3806(90)90172-U

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@article{3795d8396f204aa0af5c14293c46f1d8,

title = "The p38 and p34 polypeptides of growth cone particle membranes are the α- and β-subunits of G proteins",

abstract = "Growth cone particle (GCP) membranes prepared from fetal day 17 rat brain are comprised of 5 major polypeptides as analyzed by SDS-PAGE: tubulin (p52), actin (p42), pp46/GAP-43 and two unidentified species, p38 and p3414,46,52. Antibodies specific for the α- and β-subunits of G proteins recognize p38 and p34, respectively, on immunoblots following one- and two-dimensional electrophoretic separation. That G protein subunits comprise major species of GCP membrane-associated polypeptides suggests a role for G proteins in transmembrane signaling in nerve growth cones.",

keywords = "G protein, Growth cone, Growth cone particle, Growth cone particle membrane",

author = "Edmonds, {Brian T.} and Moomaw, {Carolyn R.} and Hsu, {Joan T.} and Slaughter, {Clive A.} and Leland Ellis",

note = "Funding Information: We wish to thank Suzi Mumby and AI Gilman for generously providing G protein specific antibodies, our colleagues in the Ellis lab for their advice and discussions, Flora Katz for many thoughtful discussions and Flora Katz and AI Gilman for their comments on the manuscript. This work was supported by the Howard Hughes Medical Institute.",

year = "1990",

month = oct,

day = "1",

doi = "10.1016/0165-3806(90)90172-U",

language = "English (US)",

volume = "56",

pages = "131--136",

journal = "Developmental Brain Research",

issn = "0165-3806",

publisher = "Elsevier BV",

number = "1",

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TY - JOUR

T1 - The p38 and p34 polypeptides of growth cone particle membranes are the α- and β-subunits of G proteins

AU - Edmonds, Brian T.

AU - Moomaw, Carolyn R.

AU - Hsu, Joan T.

AU - Slaughter, Clive A.

AU - Ellis, Leland

N1 - Funding Information: We wish to thank Suzi Mumby and AI Gilman for generously providing G protein specific antibodies, our colleagues in the Ellis lab for their advice and discussions, Flora Katz for many thoughtful discussions and Flora Katz and AI Gilman for their comments on the manuscript. This work was supported by the Howard Hughes Medical Institute.

PY - 1990/10/1

Y1 - 1990/10/1

N2 - Growth cone particle (GCP) membranes prepared from fetal day 17 rat brain are comprised of 5 major polypeptides as analyzed by SDS-PAGE: tubulin (p52), actin (p42), pp46/GAP-43 and two unidentified species, p38 and p3414,46,52. Antibodies specific for the α- and β-subunits of G proteins recognize p38 and p34, respectively, on immunoblots following one- and two-dimensional electrophoretic separation. That G protein subunits comprise major species of GCP membrane-associated polypeptides suggests a role for G proteins in transmembrane signaling in nerve growth cones.

AB - Growth cone particle (GCP) membranes prepared from fetal day 17 rat brain are comprised of 5 major polypeptides as analyzed by SDS-PAGE: tubulin (p52), actin (p42), pp46/GAP-43 and two unidentified species, p38 and p3414,46,52. Antibodies specific for the α- and β-subunits of G proteins recognize p38 and p34, respectively, on immunoblots following one- and two-dimensional electrophoretic separation. That G protein subunits comprise major species of GCP membrane-associated polypeptides suggests a role for G proteins in transmembrane signaling in nerve growth cones.

KW - G protein

KW - Growth cone

KW - Growth cone particle

KW - Growth cone particle membrane

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U2 - 10.1016/0165-3806(90)90172-U

DO - 10.1016/0165-3806(90)90172-U

M3 - Article

C2 - 2126227

AN - SCOPUS:0025180464

SN - 0165-3806

VL - 56

SP - 131

EP - 136

JO - Developmental Brain Research

JF - Developmental Brain Research

IS - 1

ER -

The p38 and p34 polypeptides of growth cone particle membranes are the α- and β-subunits of G proteins

Abstract

Keywords

ASJC Scopus subject areas

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