The properties of inducible membranes in animals and humans

Monique Bethel, Susan McDowell, Brahmananda Chitteti, Ying Hua Cheng, Brian H. Mullis, Janos P. Ertl, Tien Min G. Chu, Melissa A. Kacena, Jeffrey O. Anglen

Research output: Contribution to journalArticlepeer-review


Membranes form around spacers used in large bone defects. We studied the effect of the spacer material on the ability of the membrane to promote osteogenesis in an animal model and the gene expression patterns of membranes obtained from humans. Critical-sized osseous defects were created in the ulnae of 12 rabbits. Stainless steel (SS) or polymethymethacrylate (PMMA) spacers were inserted into the defects, and the animals healed for four or eight weeks. After sacrifice, samples of the membrane were grown in cell culture. Human membrane samples were collected for gene expression analyses. Cultures of rabbit membranes with bone marrow cells showed significantly greater alkaline phosphatase activity (PMMA) and mineral deposition (SS) than bone marrow cells alone. All membranes had elevated expression of osteoblast-related genes. Inducible membranes around orthopaedic implants likely contain active cells of the OB lineage and the spacer material may influence the healing properties of the membrane.

Original languageEnglish (US)
Pages (from-to)189-200
Number of pages12
JournalInternational Journal of Medical Engineering and Informatics
Issue number3
StatePublished - 2017
Externally publishedYes


  • Animal model
  • Bone healing
  • Masquelet membrane
  • Segmental bone defects

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Health Informatics


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