The PSGL-1-L-selectin signaling complex regulates neutrophil adhesion under flow

Anika Stadtmann, Giulia Germena, Helena Block, Mark Boras, Jan Rossaint, Prithu Sundd, Craig Lefort, Charles I. Fisher, Konrad Buscher, Bernadette Gelschefarth, Ana Urzainqui, Volker Gerke, Klaus Ley, Alexander Zarbock

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Neutrophils are recruited from the blood to sites of inflammation, where they contribute to immune defense but may also cause tissue damage. During inflammation, neutrophils roll along the microvascular endothelium before arresting and transmigrating. Arrest requires conformational activation of the integrin lymphocyte function-associated antigen 1 (LFA-1), which can be induced by selectin engagement. Here, we demonstrate that a subset of P-selectin glycoprotein ligand-1 (PSGL-1) molecules is constitutively associated with L-selectin. Although this association does not require the known lectin-like interaction between L-selectin and PSGL-1, the signaling output is dependent on this interaction and the cytoplasmic tail of L-selectin. The PSGL-1-L-selectin complex signals through Src family kinases, ITAM domain-containing adaptor proteins, and other kinases to ultimately result in LFA-1 activation. The PSGL-1-L-selectin complex-induced signaling effects on neutrophil slow rolling and recruitment in vivo demonstrate the functional importance of this pathway. We conclude that this is a signaling complex specialized for sensing adhesion under flow.

Original languageEnglish (US)
Pages (from-to)2171-2180
Number of pages10
JournalJournal of Experimental Medicine
Issue number11
StatePublished - Oct 2013
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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