An ESF-generating factor (EGF), erythropoietin (ESF) and a mixture of both were incubated with dithiothreitol (DTT, a protective reagent for sulfhydryl groups). Ten μM DTT completely prevented the production of 0.26 ± 0.01 IU of ESF by EGF but did not inactivate ESF. DTT was used to indicate the relative amounts of EGF and ESF activity in a mixture of both.
|Original language||English (US)|
|Number of pages||3|
|Journal||Proceedings of the Society for Experimental Biology and Medicine|
|State||Published - Jul 1971|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)