The Selective Inactivation of an ESF-Generating Factor (EGF) in the Presence of Erythropoietin (ESF)

Jasper P. Lewis; Emily T. Welch; W. Aubrey Neal; Russell R. Moores; William G. Lewis; Claude Starr Wright; Linda L. Smith; Coit M. Dubose

doi:10.3181/00379727-137-35666

The Selective Inactivation of an ESF-Generating Factor (EGF) in the Presence of Erythropoietin (ESF)

Jasper P. Lewis, Emily T. Welch, W. Aubrey Neal, Russell R. Moores, William G. Lewis, Claude Starr Wright, Linda L. Smith, Coit M. Dubose

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

An ESF-generating factor (EGF), erythropoietin (ESF) and a mixture of both were incubated with dithiothreitol (DTT, a protective reagent for sulfhydryl groups). Ten μM DTT completely prevented the production of 0.26 ± 0.01 IU of ESF by EGF but did not inactivate ESF. DTT was used to indicate the relative amounts of EGF and ESF activity in a mixture of both.

Original language	English (US)
Pages (from-to)	783-785
Number of pages	3
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	137
Issue number	3
DOIs	https://doi.org/10.3181/00379727-137-35666
State	Published - Jul 1971
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "The Selective Inactivation of an ESF-Generating Factor (EGF) in the Presence of Erythropoietin (ESF)",

abstract = "An ESF-generating factor (EGF), erythropoietin (ESF) and a mixture of both were incubated with dithiothreitol (DTT, a protective reagent for sulfhydryl groups). Ten μM DTT completely prevented the production of 0.26 ± 0.01 IU of ESF by EGF but did not inactivate ESF. DTT was used to indicate the relative amounts of EGF and ESF activity in a mixture of both.",

author = "Lewis, {Jasper P.} and Welch, {Emily T.} and Neal, {W. Aubrey} and Moores, {Russell R.} and Lewis, {William G.} and Wright, {Claude Starr} and Smith, {Linda L.} and Dubose, {Coit M.}",

note = "Funding Information: Supported in part by Grants HE-10591-07 (HEM), FR-0061, FR-5365, and HE-12958 from the National Institutes of Health and a grant from the Veterans Administration.",

year = "1971",

month = jul,

doi = "10.3181/00379727-137-35666",

language = "English (US)",

volume = "137",

pages = "783--785",

journal = "Proceedings of the Society for Experimental Biology and Medicine",

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T1 - The Selective Inactivation of an ESF-Generating Factor (EGF) in the Presence of Erythropoietin (ESF)

AU - Lewis, Jasper P.

AU - Welch, Emily T.

AU - Neal, W. Aubrey

AU - Moores, Russell R.

AU - Lewis, William G.

AU - Wright, Claude Starr

AU - Smith, Linda L.

AU - Dubose, Coit M.

N1 - Funding Information: Supported in part by Grants HE-10591-07 (HEM), FR-0061, FR-5365, and HE-12958 from the National Institutes of Health and a grant from the Veterans Administration.

PY - 1971/7

Y1 - 1971/7

N2 - An ESF-generating factor (EGF), erythropoietin (ESF) and a mixture of both were incubated with dithiothreitol (DTT, a protective reagent for sulfhydryl groups). Ten μM DTT completely prevented the production of 0.26 ± 0.01 IU of ESF by EGF but did not inactivate ESF. DTT was used to indicate the relative amounts of EGF and ESF activity in a mixture of both.

AB - An ESF-generating factor (EGF), erythropoietin (ESF) and a mixture of both were incubated with dithiothreitol (DTT, a protective reagent for sulfhydryl groups). Ten μM DTT completely prevented the production of 0.26 ± 0.01 IU of ESF by EGF but did not inactivate ESF. DTT was used to indicate the relative amounts of EGF and ESF activity in a mixture of both.

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The Selective Inactivation of an ESF-Generating Factor (EGF) in the Presence of Erythropoietin (ESF)

Abstract

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