TNF-α induces selectin-mediated leukocyte rolling in mouse cremaster muscle arterioles

Eric J. Kunkel, Unsu Jung, Klaus Ley

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Leukocyte roiling is commonly restricted to venules and mediated by selectins expressed both on leukocytes (L-selectin) and the vascular endothelium (P- and E-selectin). We show here that 2- to 3-h tumor necrosis factor-α (TNF-α) stimulation of the mouse cremaster muscle induces rolling in arterioles (diameters 30-70 μm; wall shear rates 225-1,770 s-1). Weak P-selectin expression was detected on arteriolar endothelium of TNF-α- stimulated cremaster muscles. No rolling was observed in arterioles smaller than 30 μm (wall shear rates 1,500-3,250 s-1). The arteriolar rolling flux fraction in wild-type mice averaged ~5% and rolling was blocked by the P- selectin monoclonal antibody (MAb) RB40.34. Rolling in L- and E-selectin- deficient mice was similar to that in wild-type mice and was also blocked by the MAb RB40.34. Rolling was completely absent in arterioles of P-selectin- deficient mice. The average rolling velocity in arterioles of wild-type and L-selectin-deficient mice was ~50 μm/s but increased to ~110 μm/s in E- selectin-deficient mice and after injection of the blocking E-selectin MAb 9A9 in wild-type mice. We conclude that TNF-α treatment induces P-selectin- dependent rolling in arterioles that requires E-selectin for rolling at normal velocities.

Original languageEnglish (US)
Pages (from-to)H1391-H1400
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume272
Issue number3 41-3
DOIs
StatePublished - Mar 1997
Externally publishedYes

Keywords

  • in vivo
  • inflammation
  • microcirculation
  • shear rate
  • tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'TNF-α induces selectin-mediated leukocyte rolling in mouse cremaster muscle arterioles'. Together they form a unique fingerprint.

Cite this