TY - JOUR
T1 - Toxicity assessment of 7 anticancer compounds in zebrafish
AU - Gao, Xiao Ping
AU - Feng, Feng
AU - Zhang, Xiao Qi
AU - Liu, Xiao Xin
AU - Wang, Yu Bin
AU - She, Jin Xiong
AU - He, Zhi Heng
AU - He, Ming Fang
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by Jiangsu Provincial Natural Science Fund (Grant BK2011796), New Teacher Fund from Chinese Ministry of Education (Grant 20123221120005), and National Natural Science Foundation of China (Grants 81172947 and 81073129).
PY - 2014
Y1 - 2014
N2 - Toxicity is one of the major reasons for failure in drug development. Zebrafish, as an ideal vertebrate model, could also be used to evaluate drug toxicity. In this study, we aimed to show the predictability and highlight novel findings of toxicity in zebrafish model. Seven anticancer compounds, including triptolide (TP), gambogic acid (GA), mycophenolic acid (MPA), curcumin, auranofin, thalidomide, and taxol, were assessed in zebrafish for their toxicity. Three compounds (GA, TP, and taxol) showed highest acute lethality, with 50% lethal concentration ≈ 1 μmol/L. Missing tails, severe pericardial edema, and enlarged yolk sacs were observed in MPA-treated embryos. The development of pectoral fins was severely disturbed in thalidomide-, GA-, and TP-treated embryos. Bradycardia was observed in MPA- and thalidomide-treated groups. Our findings suggested that the zebrafish are a good model for toxicity assessment of anticancer compounds.
AB - Toxicity is one of the major reasons for failure in drug development. Zebrafish, as an ideal vertebrate model, could also be used to evaluate drug toxicity. In this study, we aimed to show the predictability and highlight novel findings of toxicity in zebrafish model. Seven anticancer compounds, including triptolide (TP), gambogic acid (GA), mycophenolic acid (MPA), curcumin, auranofin, thalidomide, and taxol, were assessed in zebrafish for their toxicity. Three compounds (GA, TP, and taxol) showed highest acute lethality, with 50% lethal concentration ≈ 1 μmol/L. Missing tails, severe pericardial edema, and enlarged yolk sacs were observed in MPA-treated embryos. The development of pectoral fins was severely disturbed in thalidomide-, GA-, and TP-treated embryos. Bradycardia was observed in MPA- and thalidomide-treated groups. Our findings suggested that the zebrafish are a good model for toxicity assessment of anticancer compounds.
KW - acute toxicity
KW - anticancer compounds
KW - cardiovascular toxicity
KW - developmental toxicity
KW - zebrafish
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U2 - 10.1177/1091581814523142
DO - 10.1177/1091581814523142
M3 - Article
C2 - 24563414
AN - SCOPUS:84898943429
SN - 1091-5818
VL - 33
SP - 98
EP - 105
JO - International Journal of Toxicology
JF - International Journal of Toxicology
IS - 2
ER -