Toxicity effect of silver nanoparticles on photosynthetic pigment content, growth, ROS production and ultrastructural changes of microalgae chlorella vulgaris

Layla J. Hazeem, Gamze Kuku, Etienne Dewailly, Christian Slomianny, Alexandre Barras, Abderrahmane Hamdi, Rabah Boukherroub, Mustafa Culha, Mohamed Bououdina

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Silver nanoparticles (Ag NPs) exhibit antibacterial activity and are extensively used in numerous applications. The aim of this study was to examine the toxic effect of Ag NPs on the marine microalga, Chlorella vulgaris. The microalgae, at the exponential growth phase, were treated with different concentrations of Ag NPs (50 and 100 nm) for 96 h. X-Ray diffraction (XRD) results indicated that the used NPs are single and pure Ag phase with a mean crystallite size of 21 and 32 nm. Ag NPs were found to have a negative effect on viable cell concentration, a variable effect on chlorophyll a concentration, and increased ROS formation. Transmission electron microscopy (TEM) analysis revealed that Ag NPs were present inside the microalgae cells and formed large aggregates in the culture medium. Ag+ ions, in the form of AgNO3, were also assessed at higher concentrations and found to cause inhibitory effects.

Original languageEnglish (US)
Article number914
JournalNanomaterials
Volume9
Issue number7
DOIs
StatePublished - Jul 2019
Externally publishedYes

Keywords

  • Ag ions
  • Chlorella vulgaris
  • Chlorophyll a
  • Reactive oxygen species
  • Silver nanoparticles
  • Viable cells

ASJC Scopus subject areas

  • General Chemical Engineering
  • General Materials Science

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