TY - JOUR
T1 - Transcriptome profiling reveals the role of ZBTB38 knock-down in human neuroblastoma
AU - Chen, Jie
AU - Xing, Chaofeng
AU - Yan, Li
AU - Wang, Yabing
AU - Wang, Haosen
AU - Zhang, Zongmeng
AU - Yu, Daolun
AU - Li, Jie
AU - Li, Honglin
AU - Li, Jun
AU - Cai, Yafei
N1 - Funding Information:
The following grant information was disclosed by the authors: National Key R&D Program of China: 2018YFC1200201.
Funding Information:
This work was supported by the innovation team of the Scientific Research Platform in Anhui Province. The authors thank current and past members of Cai lab.
Funding Information:
This work was supported by the National Key R&D Program of China (No. 2018YFC1200201), National Natural Science Foundation of China under Grant (Nos. NSFC31372207 and 81570094) and a start-up grant from Nanjing Agricultural University (No. 804090). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
Copyright © 2019 Chen et al.
PY - 2019
Y1 - 2019
N2 - ZBTB38 belongs to the zinc finger protein family and contains the typical BTB domains. As a transcription factor, ZBTB38 is involved in cell regulation, proliferation and apoptosis, whereas, functional deficiency of ZBTB38 induces the human neuroblastoma (NB) cell death potentially. To have some insight into the role of ZBTB38 in NB development, high throughput RNA sequencing was performed using the human NB cell line SH-SY5Y with the deletion of ZBTB38. In the present study, 2,438 differentially expressed genes (DEGs) in ZBTB38 - /-SH-SY5Y cells were obtained, 83.5% of which was down-regulated. Functional annotation of the DEGs in the Kyoto Encyclopedia of Genes and Genomes database revealed that most of the identified genes were enriched in the neurotrophin TRK receptor signaling pathway, including PI3K/Akt and MAPK signaling pathway. we also observed that ZBTB38 affects expression of CDK4/6, Cyclin E, MDM2, ATM, ATR, PTEN, Gadd45, and PIGs in the p53 signaling pathway. In addition, ZBTB38 knockdown significantly suppresses the expression of autophagy-related key genes including PIK3C2A and RB1CC1. The present meeting provides evidence to molecular mechanism of ZBTB38 modulating NB development and targeted anti-tumor therapies.
AB - ZBTB38 belongs to the zinc finger protein family and contains the typical BTB domains. As a transcription factor, ZBTB38 is involved in cell regulation, proliferation and apoptosis, whereas, functional deficiency of ZBTB38 induces the human neuroblastoma (NB) cell death potentially. To have some insight into the role of ZBTB38 in NB development, high throughput RNA sequencing was performed using the human NB cell line SH-SY5Y with the deletion of ZBTB38. In the present study, 2,438 differentially expressed genes (DEGs) in ZBTB38 - /-SH-SY5Y cells were obtained, 83.5% of which was down-regulated. Functional annotation of the DEGs in the Kyoto Encyclopedia of Genes and Genomes database revealed that most of the identified genes were enriched in the neurotrophin TRK receptor signaling pathway, including PI3K/Akt and MAPK signaling pathway. we also observed that ZBTB38 affects expression of CDK4/6, Cyclin E, MDM2, ATM, ATR, PTEN, Gadd45, and PIGs in the p53 signaling pathway. In addition, ZBTB38 knockdown significantly suppresses the expression of autophagy-related key genes including PIK3C2A and RB1CC1. The present meeting provides evidence to molecular mechanism of ZBTB38 modulating NB development and targeted anti-tumor therapies.
KW - Bioinformatics analysis
KW - DEGs
KW - Neuroblastoma
KW - Transcriptome
KW - ZBTB38
UR - http://www.scopus.com/inward/record.url?scp=85060582200&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060582200&partnerID=8YFLogxK
U2 - 10.7717/peerj.6352
DO - 10.7717/peerj.6352
M3 - Article
AN - SCOPUS:85060582200
SN - 2167-8359
VL - 2019
JO - PeerJ
JF - PeerJ
IS - 1
M1 - e6352
ER -