Abstract
The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines is altered in the Helicobacter pylori-infected gastric mucosa. Therefore, we aimed to evaluate the association of functional promoter variants of transforming growth factor (TGF)-B1 and matrix metalloproteinase (MMP)-7 genes with gastritis and gastric precancerous lesions. After upper gastrointestinal endoscopy, a total of 130 rapid urease test-positive patients with nonulcer dyspepsia were examined for H. pylori infection using modified Giemsa stain and IgG anti-CagA ELISA. All patients and 200 asymptomatic controls were genotyped for TGF-B1 (-509 C>T) and MMP-7 (-181 A>G) substitutions using PCR-RFLP. The genotype and allele frequencies of TGF-B1 and MMP-7 polymorphisms did not differ between patients and controls (p > 0.05). However, the CagA-positive patients with TGF-B1 -509 T allele had higher risk for gastric atrophy (p = 0.026, odds ratio [OR] = 2.38) and lymphoid follicle development (p = 0.028, OR = 2.29). In addition, CagA-positive patients carrying MMP-7 -181 G allele had risk for lymphoid follicle formation (p = 0.027, OR = 2.30). Thus, the present study revealed significant association of functional MMP-7 and TGF-B1 gene variants toward susceptibility to H. pylori-induced precancerous gastric lesions.
Original language | English (US) |
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Pages (from-to) | 295-301 |
Number of pages | 7 |
Journal | DNA and Cell Biology |
Volume | 28 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2009 |
Externally published | Yes |
Keywords
- Adult
- Alleles
- Antigens, Bacterial
- Atrophy
- Bacterial Proteins
- Female
- Gastritis
- Genotype
- Helicobacter Infections
- Helicobacter pylori
- Humans
- Lymphoid Tissue
- Male
- Matrix Metalloproteinase 7
- Middle Aged
- Polymorphism, Single Nucleotide
- Precancerous Conditions
- Promoter Regions, Genetic
- Risk
- Sequence Deletion
- Stomach
- Stomach Neoplasms
- Transforming Growth Factor beta1
- Journal Article
- Research Support, Non-U.S. Gov't