Abstract
Addition of tromantadine after virus penetration inhibited HSV-1 induced syncytium formation and virus production in HEp-2 and VERO cells and acted additively with neutralizing antibody in blocking virus spread and cytopathology. Inhibition of syncytium formation in VERO cells infected with 0.01 pfu/cell of HSV-1 GC+ was observed at a concentration <25 μg/ml. The extent of inhibition was dependent upon the multiplicity of infection and cell type. Tromantadine inhibited a late event in HSV-1 replication which appeared to be sensitive to cycloheximide. Reversal of the inhibitory effect of tromantadine on syncytium formation required new protein synthesis. HSV-1 gB, gC, and gD were synthesized in the presence of tromantadine and could be detected on the cell surface by immunofluorescence. Tromantadine most likely inhibits a cellular process that is required for syncytium formation, such as glycoprotein processing, which occurs after the synthesis of the fusion protein but before its expression on the cell surface.
Original language | English (US) |
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Pages (from-to) | 75-85 |
Number of pages | 11 |
Journal | Antiviral Research |
Volume | 14 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1990 |
Externally published | Yes |
Keywords
- Fusion
- HSV-1
- Syncytium formation
- Tromantadine
ASJC Scopus subject areas
- Pharmacology
- Virology