TSKS concentrates in spermatid centrioles during flagellogenesis

Bingfang Xu; Zhonglin Hao; Kula N. Jha; Zhibing Zhang; Craig Urekar; Laura Digilio; Silvia Pulido; Jerome F. Strauss; Charles J. Flickinger; John C. Herr

doi:10.1016/j.ydbio.2008.03.043

TSKS concentrates in spermatid centrioles during flagellogenesis

Bingfang Xu, Zhonglin Hao, Kula N. Jha, Zhibing Zhang, Craig Urekar, Laura Digilio, Silvia Pulido, Jerome F. Strauss, Charles J. Flickinger, John C. Herr

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Centrosomal coiled-coil proteins paired with kinases play critical roles in centrosomal functions within somatic cells, however knowledge regarding gamete centriolar proteins is limited. In this study, the substrate of TSSK1 and 2, TSKS, was localized during spermiogenesis to the centrioles of post-meiotic spermatids, where it reached its greatest concentration during the period of flagellogenesis. This centriolar localization persisted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrioles are known to undergo complete degeneration. In addition to the centriolar localization during flagellogenesis, mouse TSKS and the TSSK2 kinase localized in the tail and acrosomal regions of mouse epididymal sperm, while TSSK2 was found in the equatorial segment, neck and the midpiece of human spermatozoa. TSSK2/TSKS is the first kinase/substrate pair localized to the centrioles of spermatids and spermatozoa. Coupled with the infertility due to haploinsufficiency noted in chimeric mice with deletion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an indispensable role during spermiogenesis.

Original language	English (US)
Pages (from-to)	201-210
Number of pages	10
Journal	Developmental Biology
Volume	319
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2008.03.043
State	Published - Jul 15 2008
Externally published	Yes

Keywords

Centriole
Flagellogenesis
Spermatid
Spermatozoa
Spermiogenesis
TSKS
TSSK2

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.ydbio.2008.03.043

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@article{74646e7e51c14aa9ab811fff9d81c843,

title = "TSKS concentrates in spermatid centrioles during flagellogenesis",

abstract = "Centrosomal coiled-coil proteins paired with kinases play critical roles in centrosomal functions within somatic cells, however knowledge regarding gamete centriolar proteins is limited. In this study, the substrate of TSSK1 and 2, TSKS, was localized during spermiogenesis to the centrioles of post-meiotic spermatids, where it reached its greatest concentration during the period of flagellogenesis. This centriolar localization persisted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrioles are known to undergo complete degeneration. In addition to the centriolar localization during flagellogenesis, mouse TSKS and the TSSK2 kinase localized in the tail and acrosomal regions of mouse epididymal sperm, while TSSK2 was found in the equatorial segment, neck and the midpiece of human spermatozoa. TSSK2/TSKS is the first kinase/substrate pair localized to the centrioles of spermatids and spermatozoa. Coupled with the infertility due to haploinsufficiency noted in chimeric mice with deletion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an indispensable role during spermiogenesis.",

keywords = "Centriole, Flagellogenesis, Spermatid, Spermatozoa, Spermiogenesis, TSKS, TSSK2",

author = "Bingfang Xu and Zhonglin Hao and Jha, {Kula N.} and Zhibing Zhang and Craig Urekar and Laura Digilio and Silvia Pulido and Strauss, {Jerome F.} and Flickinger, {Charles J.} and Herr, {John C.}",

note = "Funding Information: We thank Dr. Robert A. Bloodgood and others at the Center for Research in Contraceptive and Reproductive Health for critique of the manuscript. This work was supported in part by NIH R03 HD055129, R01 HD 045783, R01 HD037416, NIH Fogarty International Center Grant D43 TW/HD 00654, NIH U54 HD29099, the Andrew W. Mellon Foundation and Schering AG.",

year = "2008",

month = jul,

day = "15",

doi = "10.1016/j.ydbio.2008.03.043",

language = "English (US)",

volume = "319",

pages = "201--210",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - TSKS concentrates in spermatid centrioles during flagellogenesis

AU - Xu, Bingfang

AU - Hao, Zhonglin

AU - Jha, Kula N.

AU - Zhang, Zhibing

AU - Urekar, Craig

AU - Digilio, Laura

AU - Pulido, Silvia

AU - Strauss, Jerome F.

AU - Flickinger, Charles J.

AU - Herr, John C.

N1 - Funding Information: We thank Dr. Robert A. Bloodgood and others at the Center for Research in Contraceptive and Reproductive Health for critique of the manuscript. This work was supported in part by NIH R03 HD055129, R01 HD 045783, R01 HD037416, NIH Fogarty International Center Grant D43 TW/HD 00654, NIH U54 HD29099, the Andrew W. Mellon Foundation and Schering AG.

PY - 2008/7/15

Y1 - 2008/7/15

N2 - Centrosomal coiled-coil proteins paired with kinases play critical roles in centrosomal functions within somatic cells, however knowledge regarding gamete centriolar proteins is limited. In this study, the substrate of TSSK1 and 2, TSKS, was localized during spermiogenesis to the centrioles of post-meiotic spermatids, where it reached its greatest concentration during the period of flagellogenesis. This centriolar localization persisted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrioles are known to undergo complete degeneration. In addition to the centriolar localization during flagellogenesis, mouse TSKS and the TSSK2 kinase localized in the tail and acrosomal regions of mouse epididymal sperm, while TSSK2 was found in the equatorial segment, neck and the midpiece of human spermatozoa. TSSK2/TSKS is the first kinase/substrate pair localized to the centrioles of spermatids and spermatozoa. Coupled with the infertility due to haploinsufficiency noted in chimeric mice with deletion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an indispensable role during spermiogenesis.

AB - Centrosomal coiled-coil proteins paired with kinases play critical roles in centrosomal functions within somatic cells, however knowledge regarding gamete centriolar proteins is limited. In this study, the substrate of TSSK1 and 2, TSKS, was localized during spermiogenesis to the centrioles of post-meiotic spermatids, where it reached its greatest concentration during the period of flagellogenesis. This centriolar localization persisted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse sperm, where centrioles are known to undergo complete degeneration. In addition to the centriolar localization during flagellogenesis, mouse TSKS and the TSSK2 kinase localized in the tail and acrosomal regions of mouse epididymal sperm, while TSSK2 was found in the equatorial segment, neck and the midpiece of human spermatozoa. TSSK2/TSKS is the first kinase/substrate pair localized to the centrioles of spermatids and spermatozoa. Coupled with the infertility due to haploinsufficiency noted in chimeric mice with deletion of Tssk1 and 2 (companion paper) this centriolar kinase/substrate pair is predicted to play an indispensable role during spermiogenesis.

KW - Centriole

KW - Flagellogenesis

KW - Spermatid

KW - Spermatozoa

KW - Spermiogenesis

KW - TSKS

KW - TSSK2

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UR - http://www.scopus.com/inward/citedby.url?scp=46049119413&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2008.03.043

DO - 10.1016/j.ydbio.2008.03.043

M3 - Article

C2 - 18495105

AN - SCOPUS:46049119413

SN - 0012-1606

VL - 319

SP - 201

EP - 210

JO - Developmental Biology

JF - Developmental Biology

IS - 2

ER -

TSKS concentrates in spermatid centrioles during flagellogenesis

Abstract

Keywords

ASJC Scopus subject areas

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