Abstract
We have used a simple negative staining technique to study the structural alterations of mitochondria from biopsies of hearts subjected to the calcium paradox and treatment with diltiazem, a calcium channel blocker. A significant (P<0.05) decrease in the number of spheres on the mitochondrial membranes occurs during the calcium paradox (58.0 ±4. I/μm vs. control 80.5±6.5). Treatment with diltiazem prevented the loss of spheres from mitochondrial membranes during the calcium paradox (75.5 ±5.0 gmm). We found that this negative staining technique can be used for quick assessment of the condition of mitochondria in biopsies from normal and pathological organs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 267-272 |
| Number of pages | 6 |
| Journal | Virchows Archiv B Cell Pathology Including Molecular Pathology |
| Volume | 39 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1982 |
| Externally published | Yes |
Keywords
- ATPase
- Calcium channel blocker
- Calcium paradox
- Diltiazem
- Electron microscopy
- Mitochondria
- Negative staining
ASJC Scopus subject areas
- Pathology and Forensic Medicine
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