TY - JOUR
T1 - Upregulation of heat shock factor 1 transcription activity is associated with hepatocellular carcinoma progression
AU - Li, Shulian
AU - Ma, Wanli
AU - Fei, Teng
AU - Lou, Qiang
AU - Zhang, Yaqin
AU - Cui, Xiukun
AU - Qin, Xiaoming
AU - Zhang, Jun
AU - Liu, Guangchao
AU - Dong, Zheng
AU - Ma, Yuanfang
AU - Song, Zhengshun
AU - Hu, Yanzhong
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Heat shock factor 1 (HSF1) is associated with tissue specific tumorigenesis in a number of mouse models, and has been used a as prognostic marker of cancer types, including breast and prostatic cancer. However, its role in human hepatocellular carcinoma (HCC) is not well understood. Using immunoblotting and immunohistochemical staining, it was identified that HSF1 and its serine (S) 326 phosphorylation, a biomarker of HSF1 activation, are significantly upregulated in human HCC tissues and HCC cell lines compared with their normal counterparts. Cohort analyses indicated that upregulation of the expression of HSF1 and its phospho S326 is significantly correlated with HCC progression, invasion and patient survival prognosis (P<0.001); however, not in the presence of a hepatitis B virus infection and the expression of alpha-fetoprotein and carcinoembryonic antigen. Knockdown of HSF1 with shRNA induced the protein expression of tumor suppressor retinoblastoma protein, resulting in attenuated plc/prf5 cell growth and colony formation in vitro. Taken together, these data markedly support that HSF1 is a potential prognostic marker and therapeutic target for the treatment of HCC.
AB - Heat shock factor 1 (HSF1) is associated with tissue specific tumorigenesis in a number of mouse models, and has been used a as prognostic marker of cancer types, including breast and prostatic cancer. However, its role in human hepatocellular carcinoma (HCC) is not well understood. Using immunoblotting and immunohistochemical staining, it was identified that HSF1 and its serine (S) 326 phosphorylation, a biomarker of HSF1 activation, are significantly upregulated in human HCC tissues and HCC cell lines compared with their normal counterparts. Cohort analyses indicated that upregulation of the expression of HSF1 and its phospho S326 is significantly correlated with HCC progression, invasion and patient survival prognosis (P<0.001); however, not in the presence of a hepatitis B virus infection and the expression of alpha-fetoprotein and carcinoembryonic antigen. Knockdown of HSF1 with shRNA induced the protein expression of tumor suppressor retinoblastoma protein, resulting in attenuated plc/prf5 cell growth and colony formation in vitro. Taken together, these data markedly support that HSF1 is a potential prognostic marker and therapeutic target for the treatment of HCC.
KW - Heat shock factor 1
KW - Hepatocellular carcinoma
KW - Phosphorylation
KW - Retinoblastoma protein
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U2 - 10.3892/mmr.2014.2547
DO - 10.3892/mmr.2014.2547
M3 - Article
C2 - 25199534
AN - SCOPUS:84907210666
SN - 1791-2997
VL - 10
SP - 2313
EP - 2321
JO - Molecular Medicine Reports
JF - Molecular Medicine Reports
IS - 5
ER -