TY - JOUR
T1 - Usefulness of CD11a and CD18 in flow cytometric immunophenotypic analysis for diagnosis of acute promyelocytic leukemia
AU - Zhou, Yi
AU - Jorgensen, Jeffrey L.
AU - Wang, Sa A.
AU - Ravandi, Farhad
AU - Cortes, Jorge
AU - Kantarjian, Hagop M.
AU - Medeiros, L. Jeffrey
AU - Konoplev, Sergej
PY - 2012/11
Y1 - 2012/11
N2 - Acute promyelocytic leukemia (APL) is an aggressive disease that requires prompt diagnosis and therapy. Flow cytometry immunophenotyping can serve as a screening test for APL before the results of cytogenetic or molecular testing for t(15;17)(q22;q21)/PML-RAR a are available. CD11a and CD18 are leukocyte β2-integrin subunits expressed in most acute myeloid leukemia (AML) types, but are often dimly expressed or absent in APL. We used flow cytometry immunophenotyping with an antibody panel including CD11a and CD18 to assess 36 APL and 33 other AML cases. HLA-DR, CD11a, and CD18 were absent in 81% of APL and 12% of other AML cases (specificity, 88%). By further including combinations of HLA-DR-, CD2+, and either CD11a- or CD18-, we identified 92% of APL cases with 85% specificity. These data compare favorably with the combination of HLA-DR- , CD34-, and CD117+ for APL diagnosis, which had a sensitivity of 64% in this study.
AB - Acute promyelocytic leukemia (APL) is an aggressive disease that requires prompt diagnosis and therapy. Flow cytometry immunophenotyping can serve as a screening test for APL before the results of cytogenetic or molecular testing for t(15;17)(q22;q21)/PML-RAR a are available. CD11a and CD18 are leukocyte β2-integrin subunits expressed in most acute myeloid leukemia (AML) types, but are often dimly expressed or absent in APL. We used flow cytometry immunophenotyping with an antibody panel including CD11a and CD18 to assess 36 APL and 33 other AML cases. HLA-DR, CD11a, and CD18 were absent in 81% of APL and 12% of other AML cases (specificity, 88%). By further including combinations of HLA-DR-, CD2+, and either CD11a- or CD18-, we identified 92% of APL cases with 85% specificity. These data compare favorably with the combination of HLA-DR- , CD34-, and CD117+ for APL diagnosis, which had a sensitivity of 64% in this study.
KW - Acute progranulocytic leukemia
KW - CD11a
KW - CD18
KW - Flow cytometry
KW - β2-integrin
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UR - http://www.scopus.com/inward/citedby.url?scp=84868119408&partnerID=8YFLogxK
U2 - 10.1309/AJCPQU9R3FSLKFMI
DO - 10.1309/AJCPQU9R3FSLKFMI
M3 - Article
C2 - 23086776
AN - SCOPUS:84868119408
SN - 0002-9173
VL - 138
SP - 744
EP - 750
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 5
ER -