@inbook{9c2666ec6f424d59a4fba0623b4e7641,
title = "Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-Edited iPSC in Skeletal Muscle",
abstract = "Tumorigenesis and attendant safety risks are significant concerns of induced pluripotent stem cell (iPSC)-based therapies. Thus, it is crucial to evaluate iPSC proliferation, differentiation, and tumor formation after transplantation. Several approaches have been employed for tracking the donor cells, including fluorescent protein and luciferase, but both have limitations. Here, we introduce a protocol using iRFP genetic labeling technology to track tumor formation of iPSCs in skeletal muscle after CRISPR/Cas9 gene editing.",
keywords = "CRISPR/Cas9, Induced pluripotent stem cells (iPSC), Infrared fluorescent protein (iRFP), Tumor formation",
author = "Yue Jin and Yan Shen and Weintraub, {Neal L.} and Yaoliang Tang",
note = "Funding Information: N. Weintraub and Y. Tang were partially supported by the American Heart Association: GRNT31430008, NIH-AR070029, NIH-HL086555, and NIH-HL134354. Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
doi = "10.1007/978-1-0716-0364-2_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "73--83",
booktitle = "Methods in Molecular Biology",
}