Validation of antisperm antibody testing in frozen sera

S. Hinds, E. Eisenberg, M. P. Diamond, G. Reed

Research output: Contribution to journalArticlepeer-review


Objective: To determine whether antisperm antibody results obtained from a serum sample tested when received are comparable to the results obtained when the same serum sample is frozen and stored at -20°C before testing. Methods: Sera from 17 patients were evaluated for antisperm antibodies by immunobead binding. Each sample was tested fresh, and simultaneously an aliquot was frozen and analyzed by the same methodology at a later date. Results: No significant difference was observed in antisperm antibody binding between fresh and frozen serum samples. A significant difference was found in the variation between fresh and frozen samples if different sperm donors were used for testing the same patient. Conclusion: Results of antisperm antibody testing on frozen sera accurately reflects the presence of antisperm antibodies.

Original languageEnglish (US)
Pages (from-to)67-71
Number of pages5
JournalJournal of Gynecologic Techniques
Issue number2
StatePublished - 1998
Externally publishedYes


  • Antisperm antibodies
  • Freezing
  • Male factor infertility
  • Sperm

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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