Abstract
A DNA microarray containing all Singapore grouper iridovirus (SGIV) open reading frames (ORFs) was constructed to map the viral gene transcriptional profiles in virus-infected grouper spleen (GS) cells and in spleen tissues of virus-infected grouper. The results showed that viral genes started to be transcribed as early as 1 h postinfection (p.i.), and followed by a rapid increasing gene expression along with virus infection in cell cultures. The three temporal kinetic classes (15 immediate-early, 89 early and 53 late transcripts) were classified during an in vitro infection by their dependence on de novo protein synthesis and viral DNA replication inhibitors. In SGIV-infected grouper, Epinephulus coioides, most of the viral genes were expressed between 1 and 4 d p.i., and the number and expression levels started to decrease after 5 d p.i. These data were confirmed by real-time RT-PCR. This study provides an experimental basis for investigation of virus-host interactions and the development of control strategies against SGIV infection.
Original language | English (US) |
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Pages (from-to) | 39-48 |
Number of pages | 10 |
Journal | Virology |
Volume | 377 |
Issue number | 1 |
DOIs | |
State | Published - Jul 20 2008 |
Externally published | Yes |
Keywords
- Expression pattern
- Grouper
- SGIV
- Temporal kinetic class
- Transcriptional profiles
- cDNA microarrays
ASJC Scopus subject areas
- Virology