ANALYSIS OF LYMPHOPOIESIS IN ANEMIA

The goal of the proposed study is to obtain training and experience in
immunological research while performing experiments designed to elucidate
the interaction of anemia and lymphoid cell perturbations in the ha/ha
mutant mouse. Evaluation of this animal model will provide the opportunity
for a broadened research environment which can be used to focus on the
question of altered lymphopoiesis. Research plan will allow development of
expertise in new areas: (1) labeling studies to dissect cellular kinetics
(2) use of specific antisera and flow cytometry to analyze subpopulations
of cells (3) use of transplantation studies to examine any relationship
between erythropoiesis and lymphopoiesis and (4) studies to evaluate the
role of NK cells in hemopoietic regulation. Any one of these aspects could
offer material for future studies. These experiments will be carried out
in laboratories where the techniques are used routinely and under the
supervision of well-established individuals in the field. Characterization
of this mouse model may provide a resource for studies involving normal
lymphopoiesis and the relationship between the immunologic and
hematopoietic system.